Chordicoccus furentiruminis, gen. nov., sp. nov., a novel succinic acid producing bacterium isolated from a steer on a high grain diet. Issue 3 (1st March 2023)

• Record Type: Journal Article
• Authors: Gaffney, James; Embree, Jordan; Gilmore, Sean; Embree, Mallory
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 73:Issue 3(2023)
• Publication Date: 2023-03-01
• Subjects: Chordicoccus -- genome -- rumen; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005751
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : This study presents MP1D12 T (=NRRL B-67553 T =NCTC 14480 T ), an isolate from the ruminal content of an Angus steer fed a high grain diet. Phenotypic and genotypic traits of the isolate were explored. MP1D12 T was found to be a strictly anaerobic, catalase-negative, oxidase-negative, coccoid bacterium that frequently grows in chains. Analysis of metabolic products as a result of carbohydrate fermentation showed succinic acid as the major organic acid produced with lactic acid and acetic acid as minor products. Phylogenetic analysis of MP1D12 T based on 16S rRNA nucleotide sequence and amino acid sequences from the whole genome presents a divergent lineage from other members in the family Lachnospiraceae . 16S rRNA sequence comparison, whole genome average nucleotide identity digital DNA–DNA hybridization and average amino acid identity results suggest that MP1D12 T represents a novel species in a novel genus within the family Lachnospiraceae . We propose the creation of the genus Chordicoccus in which MP1D12 T represents the type strain for the novel species Chordicoccus furentiruminis .