Bacillomycin D with calcium propionate effectively inhibited microbial growth and reduced deoxynivalenol on maize kernels during storage. (March 2023)

• Record Type: Journal Article
• Authors: Sun, Jing; Zhou, Yan; Lu, Zhaoxin; Lu, Yingjian
• Is Part Of: Journal of stored products research. Volume 101(2023)
• Publication Date: 2023-03
• Subjects: Bacillomycin D -- Calcium propionate -- Deoxynivalenol -- Maize kernels; Food -- Storage -- Periodicals; Farm produce -- Storage -- Diseases and injuries -- Periodicals; Entomology -- Periodicals; Food Contamination -- Periodicals; Food Preservation -- Periodicals; Insect Control -- Periodicals; Aliments -- Entreposage -- Périodiques; Produits agricoles -- Entreposage -- Maladies et dommages -- Périodiques; Electronic journals; 631.568
• DOI: 10.1016/j.jspr.2022.102070
• Languages: English
• ISSNs: 0022-474X

Abstract

Abstract: Maize kernels contaminated with deoxynivalenol (DON) reduced their nutritional and economic values and were exposed to greater risks under high moisture conditions. In this study, an antifungal combination of bacillomycin D (BD) with calcium propionate (CP) was used in maize polluted by microorganisms and DON. During the storage of maize kernels, BD combined with calcium propionate (BD+CP) treatment inhibited the growth of fungi and bacteria. High dose (250 μg/g BD and 1800 μg/g CP) treatments reduced fungal growth to less than 4 lg CFU/g, while BD+CP groups with 14% and 16% moisture content both maintained bacterial counts below 4 lg CFU/g at the end of storage. High-throughput sequencing results showed that the main contaminating fungi in the samples were Fusarium spp. with a percentage of 32.52%, and the average value of initial DON content in the samples was 210.49 ng/g. The high-dose treatment groups of BD+CP almost completely inhibited the production of DON with 217.99 ± 8.07–247.33 ± 53.34 ng/g ( P < 0.05 ). Furthermore, the production of free fatty acid values (FAV), and the decomposition of protein components were also controlled. BD+CP treatment could effectively inhibit the growth of microorganisms in maize kernels during storage, slow the production of DON, and prolong the shelf life of maize kernels. These results showed that BD+CP could be applied to the storage of naturally contaminated grain, and it is a broad-spectrum fungicide combination with practical application value. Highlights: Maize samples were mainly contaminated with Fusarium by high-throughput sequencing. Bacillomycin D and calcium propionate inhibited DON production and nutrient decomposition. Combination of bacillomycin D and calcium propionate facilitated maize kernels storage.