The effect of TiO2NPs on cloransulam-methyl toxicity to earthworm (Eisenia fetida). (May 2023)

• Record Type: Journal Article
• Authors: Wu, Ruolin; Liu, Hunan; Lu, Chengbo; Hou, Kaixuan; Wang, Xiaole; Wang, Jun; Du, Zhongkun; Li, Bing; Zhu, Lusheng; Wang, Jinhua
• Is Part Of: Chemosphere. Volume 322(2023)
• Publication Date: 2023-05
• Subjects: Antioxidant enzyme -- Gene expression -- Oxidative stress -- Co-exposure -- Integrated biomarker response; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2023.138242
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Cloransulam-methyl is a new herbicide and has broad application prospect. However, the effect of cloransulam-methyl on earthworm have yet to be clarified. As more and more titanium dioxide nanoparticles (TiO2 NPs) enter the soil, cloransulam-methyl and TiO2 NPs have a risk of co-exposure, but the effect of TiO2 NPs on cloransulam-methyl toxicity is unknown. In the study, the ecotoxicity of cloransulam-methyl (0.1, 1 mg kg −1 ) on earthworm and the effect of TiO2 NPs (10 mg kg −1 ) on cloransulam-methyl toxicity was investigated after exposure for 28 and 56 d. Exposure tests showed cloransulam-methyl and cloransulam-methyl + TiO2 NPs promoted the accumulation of reactive oxygen species, malondialdehyde and 8-hydroxydeoxyguanosine, increased the activities of superoxide dismutase and catalase, resulted in lipid peroxidation and DNA damage. Besides, the results at the genetic level showed cloransulam-methyl and cloransulam-methyl + TiO2 NPs altered the expression of physiologically-related genes, which demonstrated that cloransulam-methyl and cloransulam-methyl + TiO2 NPs induced oxidative stress and cell apoptosis, and disturbed the normal reproduction in earthworm. The results of comprehensive toxicity comparison indicated cloransulam-methyl and TiO2 NPs co-exposure has higher toxicity compared to cloransulam single exposure. Our results suggest that TiO2 NPs can enhance the toxicity of cloransulam-methyl on Eisenia fetida in terms of oxidative stress, cell apoptosis and reproduction aspects. Based on above studies, it is of great importance for evaluating the risk of cloransulam-methyl co-exposure with TiO2 NPs in soil. Graphical abstract: Image 1 Highlights: Cloransulam-methyl induces oxidative stress and cell apoptosis on E. fetida. Cloransulam-methyl disturbs gene expressions related to reproduction. The Hsp70 and ANN genes are more sensitive to cloransulam-methyl exposure. TiO2 NPs enhances the cloransulam-methyl toxicity on E. fetida.