Radial flow tubular membrane bioreactor for enhanced enzymatic hydrolysis of lignocellulosic waste biomass. (1st June 2023)

• Record Type: Journal Article
• Authors: Al-Mardeai, Saleha; Elnajjar, Emad; Hashaikeh, Raed; Kruczek, Boguslaw; Van der Bruggen, Bart; Al-Zuhair, Sulaiman
• Is Part Of: Fuel. Volume 341(2023)
• Publication Date: 2023-06-01
• Subjects: Enzymatic hydrolysis -- Kinetic model -- Membrane bioreactor -- Product inhibition -- Product separation; DSs Date Seeds -- MBR Membrane Bioreactor -- MWCO Molecular Weight Cut-Off -- PES Polyethersulfone -- kEP2 Backward Rate Constants for the Competitive Inhibition -- kc2 Backward Rate Constant for the Reversible Esc Intermediate Formation -- kx2 Backward Rate Constant for the Reversible Non-Productive EsxIntermediate Formation -- [ESc] Concentration of the Enzyme- Hydrolyzable Substrate Complex -- [ESx] Concentration of the Enzyme- Non-Hydrolyzable Substrate Complex -- [P] Concentration of the Glucose -- P % Change in the Total Glucose Produced -- E Enzyme -- EP Enzyme-Product Complex -- kc1 Forward Rate Constant for the Reversible Esc Intermediate Formation -- kx1 Forward Rate Constants for the Reversible Non-Productive EsxIntermediate Formation -- kEP1 Forward Rate Constants for the Competitive Inhibition of the Product -- h Height -- Sc Hydrolyzable Substrate -- φ Non-Hydrolyzable Fraction Coefficient -- Sx Non-Hydrolyzable Substrate -- τ Residence Time -- σ Substrate Accessibility Coefficient -- G Total Amount of Reducing Sugars Produced -- t Time -- V Volume of the Reaction Cell; Fuel -- Periodicals; Coal -- Periodicals; Coal; Fuel; Periodicals; 662.6
• DOI: 10.1016/j.fuel.2023.127648
• Languages: English
• ISSNs: 0016-2361

Abstract

Highlights: Tubular membrane bioreactor was tested for simultaneous product separation. The design allowed handling high solid loadings with minimized mixing problems. Substrate inhibition was encountered due to the structural nature of the biomass. Increasing flowrate from 0.4 to 1.2 mL/min increased the yield up to 42.7 % A model was developed considering product separation and biomass structural changes. Abstract: A tubular membrane bioreactor was used for enhanced enzymatic hydrolysis of waste biomass, palm date seeds. The bioreactor successfully handled high solid loadings with effective simultaneous product removal. The effects of substrate concentrations, 14.3, 21.4, and 39.6 g/L, and water flowrates, 0.4, 0.8, and 1.2 mL/min, on the production of total reducing sugars were examined. At substrate concentration of 21.4 g/L, the total reducing sugars yield increased from 28.9 % to 42.7 % after 8 h as the water flowrate increased from 0.4 to 1.2 mL/min. Substrate inhibition was observed at the highest tested substrate concentration of 39.6 g/L. A dynamic model was developed to describe the designed bioreactor, accounting for the simultaneous product separation and the dynamic changes in substrate structure. The nonhydrolyzable substrate fraction parameter was found to significantly affect the model prediction. The novel membrane bioreactor and the developed model enable the industrial valorization of lignocellulosic biomass for bioethanol production.