Enhanced inactivation of Escherichia coli by ultrasound combined with peracetic acid during water disinfection. (May 2023)

• Record Type: Journal Article
• Authors: Bai, Yun; Shi, Chunhai; Zhou, Yuanhang; Zhou, Yingying; Zhang, Haocheng; Chang, Ruiting; Hu, Xueli; Hu, Jiawei; Yang, Chuanyao; Peng, Kedi; Xiang, Ping; Zhang, Zhi
• Is Part Of: Chemosphere. Volume 322(2023)
• Publication Date: 2023-05
• Subjects: Ultrasound -- E. coli -- Efficiency -- Influencing factors -- Mechanism; TEM transmission electron microscope -- SEM scanning electron microscopy -- LSCM laser scanning confocal microscopy -- PBS phosphate buffer saline -- DNA deoxyribonucleic acid -- DR6000 UV DR6000 ultraviolet spectrophotometer -- ATP adenosine triphosphate -- E. coli Escherichia coli -- PAA peracetic acid -- PI propidium iodide -- FDA fluorscein diacetate -- DBPs disinfection by-products -- HA humic acid; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2023.138095
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Peracetic acid (PAA) is a desirable disinfectant for municipal wastewater because of its potent disinfection performance and limited toxic by-products. This study explored the efficiency and mechanism of Escherichia coli inactivation by PAA combined with ultrasound simultaneously (ultrasound + PAA) or (ultrasound → PAA) sequentially. The result showed that 60 kHz ultrasound combined with PAA sequentially (60 kHz → PAA) had excellent inactivation performance on E. coli, up to 4.69-log10 . The result also showed that the increase of pH and humic acid concentration in solution significantly reduced the inactivation efficiency of 60 kHz → PAA treatment. We also observed that the increase of temperature was beneficial to the disinfection, while anions ( C l − ; H C O 3 − ) had little effect. With 60 kHz → PAA, the PAA and the synergism between PAA and ultrasound played major contribution to the inactivation, which we assumed might be due to both the diffusion of PAA into the cells and the damage to the cytomembrane by ultrasound, as evidenced through the laser confocal microscopy (LSCM), scanning electron microscope (SEM) and transmission electron microscope (TEM). The inactivation mechanism involved the destruction of cell membrane and loss of intracellular material. Empirically, 60 kHz → PAA was found to be effective for the inactivation of E. coli in actual wastewater, and the regrowth potential of E. coli treated by 60 kHz → PAA was significantly lower than that treated only by PAA. Graphical abstract: Image 1 Highlights: The inactivation efficiency of 60 kHz .→ PAA treatment is highest (4.69-log) among all treatments. PAA and 60 kHz ultrasound play excellent synergistic role in bacteria inactivation. Inactivation mechanism involved damage of the cytomembrane and loss of cell contents in the 60 kHz .→ PAA treatment. 60 kHz .→ PAA was effective for the inactivation of E. coli in real wastewater.