COL3A1: Potential prognostic predictor for head and neck cancer based on immune‐microenvironment alternative splicing. (29th August 2022)

• Record Type: Journal Article
• Authors: Shen, Yuchen; Li, Xinyu; Wang, Deming; Zhang, Liming; Li, Xiao; Su, Lixin; Fan, Xindong; Yang, Xitao
• Is Part Of: Cancer medicine. Volume 12:Number 4(2023)
• Page Start: 4882
• Page End: 4894
• Publication Date: 2022-08-29
• Subjects: alternative splicing -- COL3A1 -- HNSCC -- immune checkpoint -- prognosis; 616.994005
• DOI: 10.1002/cam4.5170
• Languages: English
• ISSNs: 2045-7634

Abstract

Abstract: We aimed to identify a novel prognostic biomarker for head and neck squamous cell carcinoma (HNSCC) based on tumor immunology‐related alternative splicing (AS). Data for 502 HNSCC and 44 normal samples were obtained from the TCGA database and used to establish an AS‐related risk model through univariate, least absolute shrinkage, and selection operator Cox regression analyses. Fresh HNSCC and normal oral tissues were surgically obtained from 44 HNSCC patients. Western blotting and quantitative reverse transcription‐PCR were used to assess gene expression levels. Kaplan–Meier was performed to evaluate patients' overall survival (OS) rate. The CIBERSORT algorithm, single‐sample gene set enrichment analysis, and immune checkpoint analyses were performed to compare immune activities between subgroups. The risk model was established using 10 pivotal AS events first. Collagen Type III Alpha 1 Chain ( COL3A1 ) were screened based on |log2FC| ≥ 1 and FDR < 0.05 criteria. COL3A1 expression levels in HNSCC tissues were elevated relative to normal tissues ( p < 0.001). Moreover, COL3A1 was a reliable biomarker for HNSCC patients' prognostic prediction in both cohorts ( p < 0.001, p = 0.0085, respectively). COL3A1 protein ( p = 0.0054) and mRNA ( p < 0.0001) levels were correlated with HNSCC differentiation. Furthermore, the T stage was correlated with COL3A1 expression ( p = 0.043), and COL3A1 expression was an independent prognostic predictor for HNSCC patients ( p = 0.006). Compared with the risk model, COL3A1 was better at evaluating immune cell infiltrations, immune activities, and immune checkpoint gene expressions of HNSCC lesions. Abstract : Expression levels of COL3A1 were correlated with HNSCC differentiation but were found to be independent factors affecting HNSCC patients CloseCurlyQuote; overall survival rates. #10;COL3A1 exhibited good performance in evaluating immune cell infiltrations, immune activities, and immune checkpoint gene expressions in HNSCC.