Influence of Defatting and Pasteurization on Nutrients and Oxidative Stress Markers in Human Milk. (May 2023)

• Record Type: Journal Article
• Authors: D'Alessandro, Annamaria; Pastore, Anna; Amadio, Patrizia; D'Agostini, Matteo; Terreri, Sara; Carsetti, Rita; Argentieri, Marta; Bernaschi, Paola; Onetti Muda, Andrea; Porzio, Ottavia; Dotta, Andrea; Salvatori, Guglielmo
• Is Part Of: Journal of human lactation. Volume 39:Number 2(2023)
• Page Start: 278
• Page End: 287
• Publication Date: 2023-05
• Subjects: antioxidant -- breastfeeding -- defatting procedure -- human milk—biochemistry -- IgA -- lactation -- macronutrients -- micronutrients -- oxidative stress markers -- prospective cross-sectional observational study; Breastfeeding -- Periodicals; Lactation -- Periodicals; 612.664
• DOI: 10.1177/08903344231156894
• Languages: English
• ISSNs: 0890-3344

Abstract

Background: It is well known that the best nutritional option for infants is human milk, and that when breastfeeding is not possible, human milk banks are a possible alternative. However, in the case of infants with fat transport disorder like chylothorax, defatting of human milk is mandatory. Research Aim: The aim of the study was to reduce milk fat content without reducing other nutrients, increasing oxidative stress, or introducing harmful microorganisms. Methods: In this prospective, cross-sectional, observational study, we examined the influence of defatting and pasteurization of 50 donor samples on fat, macro- and micronutrients, as well as on oxidative stress markers. Results: Low-temperature centrifugation proved to be very efficient in defatting, reducing the concentration of triglycerides by 85% and cholesterol by 50%. The macronutrients (proteins, albumin, and Immunoglobulin A) did not undergo significant changes due to defatting and pasteurization procedures, while iron decreased by 36%. However, as the majority of iron is retained, this result does not remarkably change the milk composition. Furthermore, oxidative stress markers and antioxidant levels were unchanged, and the milk result was microbiologically safe. Conclusions: Cold milk centrifugation proved to be an effective technique that allows the reduction of human milk lipids. The determination of triglycerides and cholesterol can be used as an indicator of skimming. This procedure is not accompanied by substantial modifications of other components present in the milk.