Water-soluble nickel (II) Schiff base complexes: Synthesis, structural characterization, DNA binding affinity, DNA cleavage, cytotoxicity, and computational studies. (4th March 2023)

• Record Type: Journal Article
• Authors: Dehghani, Fatemeh Sadat; Kalantari, Razieh; Rastegari, Banafsheh; Asadi, Zahra
• Is Part Of: Nucleosides, nucleotides & nucleic acids. Volume 42:Number 3(2023)
• Page Start: 234
• Page End: 261
• Publication Date: 2023-03-04
• Subjects: Schiff base complex -- intercalation -- molecular docking -- cytotoxicity -- DNA cleavage; Nucleosides -- Periodicals; Nucleotides -- Periodicals; Nucleic acids -- Periodicals; 572.8
• DOI: 10.1080/15257770.2022.2121838
• Languages: English
• ISSNs: 1525-7770

Abstract

Abstract: Two water-soluble nickel (II) Schiff base complexes were prepared and their interaction with fish sperm DNA (FS-DNA) was investigated by various methods including UV–vis spectroscopy, fluorescence spectroscopy, cyclic voltammetry, and viscometric measurements. Complex 1: [N, N′-bis{5-[(triphenyl phosphonium chloride)-methyl] salicylidine}-3, 4-diaminobenzophenone]nickel(II) perchloride dihydrate: [Ni(5-CH2 PPh3 -3, 4-salophen)] (ClO4 )2 .2 H2 O was synthesized as a new complex and characterized by elemental analysis, IR, 1 H NMR, thermal gravimetric analysis (TGA) and UV–vis spectroscopy. Complex 2: sodium [(N, N′-bis(5-sulfosalicyliden)-3, 4-diaminobenzophenone)aqua] nickel(II) hydrate: Na2 [Ni (5-SO3 -3, 4-salbenz)(H2 O)]. H2 O was already synthesized by our research team, but in this study, its function as a DNA-binding compound was tested, and compared with the results of complex 1-DNA binding. The calculation of different constants using absorption and emission data, all confirmed the stronger binding ability of complex 1 than complex 2 with DNA. Different thermodynamic parameters showed the interactions between DNA and complexes were the type of hydrophobic interaction for complex 1 and electrostatic interaction for complex 2. Also, the negative values of free energy changes proved a spontaneous DNA binding process. Based on cell toxicity assay against two different cell lines including Jurkat and MCF-7, the effect of complex 1 was comparable to cisplatin, and the toxicity mechanism was further justified by bright field microscopy, flow cytometry, and cleavage of DNA in the presence of H2 O2 . Besides, the docking calculations suggested intercalation after measuring the lowest-energy between the complexes and DNA. For both complexes, all analytical, spectroscopic, and molecular modeling methods supported partial intercalation as the main binding mode between the complexes and DNA.