Effect of IL-6 -174G/C and -572G/C variants on susceptibility to osteoarthritis in Turkish population. (2nd January 2023)

• Record Type: Journal Article
• Authors: Yigit, Serbulent; Tekcan, Akin; Inanir, Ahmet; Nursal, Ayse Feyda; Akkanat, Songul; Tural, Ercan
• Is Part Of: Nucleosides, nucleotides & nucleic acids. Volume 42:Number 1(2023)
• Page Start: 65
• Page End: 76
• Publication Date: 2023-01-02
• Subjects: Osteoarthritis -- interleukin-6 gene -- variant -- PCR -- RFLP; Nucleosides -- Periodicals; Nucleotides -- Periodicals; Nucleic acids -- Periodicals; 572.8
• DOI: 10.1080/15257770.2022.2107219
• Languages: English
• ISSNs: 1525-7770

Abstract

Abstract: Osteoarthritis (OA) is a complex disorder characterized by degenerative articular cartilage in which inflammatory mechanisms play a major role in the pathogenesis. Interleukin-6 (IL6), a multifunctional cytokine, can trigger osteoclast differentiation and bone resorption. Our purpose in this study was to evaluate the association of IL-6 −174 G/C (rs1800795) and −572 G/C (rs1800796) variants with the susceptibility to OA. One hundred fifty OA patients and 150 healthy individuals were enrolled in the study. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) was used for genotyping the IL-6 gene variants. The results of analyses were evaluated for statistical significance. The pain intensity was assessed using the Visual Analogue Scale (VAS). There was a statistically significant difference in the genotype and allele frequencies of the IL-6 −174 G/C variant between patients with OA and control groups ( p = 0.001, p = 0.002, respectively). IL-6 −174 G/C GG genotype and G allele were more prevalent in patients with OA. We found that the IL-6 –572 G/C variant was not different between patients and controls in either genotype distribution and allele frequency. IL-6 174 G/C and −572 G/C loci GG-GG combined genotype was significantly higher in OA patients ( p = 0.00 ). Our study suggests that there was a strong association between the IL-6 −174 G/C variant and OA in the Turkish population. Further studies on populations of different ethnic background are necessary to prove the association of IL-6 variants with OA.