Cuprous oxide–demethyleneberberine nanospheres for single near-infrared light-triggered photoresponsive-enhanced enzymatic synergistic antibacterial therapy. Issue 8 (1st February 2023)

• Record Type: Journal Article
• Authors: Wang, Tao; Yang, Xiao-Chan; Ding, Yong; Zhang, Yu-Jiao; Ru, Yu-Qing; Tan, Jia-Jun; Xu, Fang; Gao, Wei-Wei; Xia, Ya-Mu
• Is Part Of: Journal of materials chemistry. Volume 11:Issue 8(2023)
• Page Start: 1760
• Page End: 1772
• Publication Date: 2023-02-01
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/d2tb02594a
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : In this work, novel Cu2 O–DMB nanospheres are developed for NIR-enhanced enzymatic synergistic antibacterial therapy. The bactericidal rate is calculated to be as high as 99.4% against MRSA and 99.8% against AREC, with a high wound healing efficiency. Abstract : In this work, novel cuprous oxide–demethyleneberberine (Cu2 O–DMB) nanomaterials are successfully synthesized for photoresponsive-enhanced enzymatic synergistic antibacterial therapy under near-infrared (NIR) irradiation (808 nm). Cu2 O–DMB has a spherical morphology with a smaller nanosize and positive ζ potential, can trap bacteria through electrostatic interactions resulting in a targeting function. Cu2 O–DMB nanospheres show both oxidase-like and peroxidase-like activities, and serve as a self-cascade platform, which can deplete high concentrations of GSH to produce O2 ˙ − and H2 O2, then H2 O2 is transformed into ˙OH, without introducing exogenous H2 O2 . At the same time, Cu2 O–DMB nanospheres become photoresponsive, producing 1 O2 and having an efficient photothermal conversion effect upon NIR irradiation. The proposed mechanism is that the generated ROS (O2 ˙ −, ˙OH and 1 O2 ) and hyperthermia can have synergetic effects for killing bacteria. Moreover, hyperthermia is not only beneficial for destroying bacteria, but also effectively enhances the efficiency of ˙OH production and accelerates GSH oxidation. Upon NIR irradiation, Cu2 O–DMB nanospheres exhibit excellent antibacterial ability against methicillin-resistant Staphylococcus aureus ( MRSA ) and ampicillin-resistant Escherichia coli ( AREC ) with low cytotoxicity and bare bacterial resistance, destroy the bacterial membrane causing an efflux of proteins and disrupt the bacterial biofilm formation. Animal experiments show that the Cu2 O–DMB + NIR group can efficiently treat MRSA infection and promote wound healing. These results suggest that Cu2 O–DMB nanospheres are effective materials for combating bacterial infections highly efficiently and to aid the development of photoresponsive enzymatic synergistic antibacterial therapy.