A multifunctional black phosphorus nanosheet-based immunomagnetic bio-interface for heterogeneous circulating tumor cell capture and simultaneous self-identification in gastric cancer patients. Issue 8 (26th January 2023)

• Record Type: Journal Article
• Authors: Zuo, Yifan; Xia, Yi; Lu, Wenwen; Li, Yue; Xiao, Yang; Gao, Shuai; Zhou, Zhiyi; Xu, Hao; Feng, Xingqing; Li, Chenglin; Yu, Yanyan
• Is Part Of: Nanoscale. Volume 15:Issue 8(2023)
• Page Start: 3872
• Page End: 3883
• Publication Date: 2023-01-26
• Subjects: Nanoscience -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/d2nr04277k
• Languages: English
• ISSNs: 2040-3364

Abstract

Abstract : A dual-labeled fluorescent immunomagnetic nanoprobe (BP–Fe3 O4 –AuNR/Apt) was fabricated for high-performance CTC capture and rapid self-identification simultaneously with high accuracy. Abstract : A single epithelial cell adhesion molecule (EpCAM) for circulating tumor cell (CTCs) isolation has been proved to be low in efficiency as it fails to recognize EpCAM-negative CTCs. Meanwhile, the current immunocytochemical (ICC) identification strategy for the captured cells is tedious and time-consuming. To address these issues, we designed a dual-labeled fluorescent immunomagnetic nanoprobe (BP–Fe3 O4 –AuNR/Apt), by loading magnetic Fe3 O4 nanoparticles and gold nanorods (AuNRs) onto black phosphorus (BP) nanosheets and then linking them with Cy3-labeled EpCAM and Texas red-labeled tyrosine protein kinase 7 (PTK7) aptamers, which created a high-performance bio-interface for efficient, heterogeneous CTC capture and rapid self-identification with high accuracy. As few as 5 CTCs could be captured from 1.0 mL PBS, mixed cell solution and lysed blood. What's more, the presence of BP and AuNRs on this capturing interface also allowed us to preliminarily investigate the potential photothermal therapeutic effect of the probe toward CTC elimination. The applicability of the probe was further demonstrated in gastric cancer patients. By detecting the number of CTCs in the blood of gastric cancer patients, the correlations between the CTC number and the disease stage, as well as distant metastasis were systematically explored.