Elite controller CD8+ T cells exhibit comparable viral inhibition capacity, but better sustained effector properties compared to chronic progressors. Issue 6 (12th July 2016)

• Record Type: Journal Article
• Authors: Shasha, David; Karel, Dan; Angiuli, Olivia; Greenblatt, Adam; Ghebremichael, Musie; Yu, Xu; Porichis, Filippos; Walker, Bruce D
• Is Part Of: Journal of leukocyte biology. Volume 100:Issue 6(2016)
• Page Start: 1425
• Page End: 1433
• Publication Date: 2016-07-12
• Subjects: HIV control -- polyfunctionality -- apoptosis -- IL-15; Leucocytes -- Periodicals; Reticulo-endothelial system -- Periodicals; 571.96
• DOI: 10.1189/jlb.4A0915-422R
• Languages: English
• ISSNs: 0741-5400

Abstract

Abstract : Superior inhibition of viral replication by CD8 + T cells is associated with better retention of functional qualities ex-vivo, and is enhanced by IL-15. Abstract: Mechanisms modulating HIV-specific CD8+ T cell-mediated viral inhibition are not well defined. To delineate features of effective control, we compared the ability of CD8 + T cells from HIV ECs and CPs to inhibit HIV ex vivo. ECs showed superior inhibition compared to HAART-treated or untreated CPs in a typical VIA in which CD8+ T cells are rested 3 d before use ( P = 0.025). In contrast, comparable antiviral activity was observed in freshly thawed cells. Rested CD8 + T cells underwent apoptosis with preferential loss of HIV-specific cells. EC CD8 + T cells showed greater capacity to sustain polyfunctionality ex vivo compared with those of CPs, and incubation of CD8 + T cells with IL-15 augmented inhibition. These results indicate that superior ex vivo inhibition of viral replication by CD8 + T cells from ECs is associated with enhanced retention of functional qualities and that in vitro antiviral function is enhanced by IL-15.