Design and synthesis of atorvastatin derivatives with enhanced water solubility, hepatoselectivity and stability. Issue 1 (9th November 2022)

• Record Type: Journal Article
• Authors: Maklakova, Svetlana Yu.; Lopukhov, Anton V.; Khudyakov, Alexandr D.; Kovalev, Sergey V.; Mazhuga, Maria P.; Chepikova, Olga E.; Zamyatnin, Andrey A.; Majouga, Alexander G.; Klyachko, Natalia L.; Beloglazkina, Elena K.
• Is Part Of: RSC medicinal chemistry. Volume 14:Issue 1(2023)
• Page Start: 56
• Page End: 64
• Publication Date: 2022-11-09
• Subjects: Pharmaceutical chemistry -- Periodicals; 615.19005
• DOI: 10.1039/d2md00119e
• Languages: English
• ISSNs: 2632-8682

Abstract

Abstract : Atorvastatin conjugates targeting the galactose-specific hepatic asialoglycoprotein receptor with improved stability are reported. Abstract : Statins are effective 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-R) inhibitors, which are successfully used for cardiovascular disease treatment. Statins' side effects are generally attributed to poor bioavailability and hepatoselectivity, which are closely related to their high lipophilicity. Targeted delivery of statins to the liver is considered as a way to reduce unwanted side effects. Herein we report on synthesis and evaluation of atorvastatin conjugates targeting the galactose-specific hepatic asialoglycoprotein receptor (ASGPR). The prepared conjugates showed greater water solubility compared to unmodified atorvastatin. The synthesised compounds demonstrated potent binding to the ASGPR with submicromolar K D values. The conjugates with an amide bond connecting atorvastatin and the targeting moiety displayed the optimal stability under model conditions, as they underwent hydrolysis only when incubated with the intracellular protease. The hydrolysis products effectively inhibited HMG-R activity. The results suggest that the designed amide-based compounds have the potential to be further developed as orally administered prodrugs of atorvastatin.