3D printing of 'green' thermo-sensitive chitosan-hydroxyapatite bone scaffold based on lyophilized platelet-rich fibrin. (27th February 2023)

• Record Type: Journal Article
• Authors: Sui, Xin; Zhang, Huili; Yao, Jingjing; Yang, Liuqing; Zhang, Xiao; Li, Lingfeng; Wang, Jue; Li, Meihui; Liu, Zhihui
• Is Part Of: Biomedical materials. Volume 18:Number 2(2023)
• Publication Date: 2023-02-27
• Subjects: 3D printing -- bone scaffold -- chitosan -- hydroxyapatite -- lyophilized PRF; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1088/1748-605X/acbad5
• Languages: English
• ISSNs: 1748-6041

Abstract

Abstract: The critical bone defect is still an urgent problem in the field of bone repair. Here, we reported a new type of chitosan (CS)–hydroxyapatite (HAP) scaffolds based on lyophilized platelet-rich fibrin (L-PRF) for releasing abundant growth factors to realize their respective functions. It also has strong mechanical properties to maintain the stability of the bone repair environment. However, acid-soluble CS hydrogels often contain toxic and organic solvents. Moreover, chemical agents may be used for cross-linking for better mechanical properties, further increasing cytotoxicity. In this study, we used an alkali/urea dissolution system to dissolve CS, which improved its mechanical properties and made it thermo-sensitive. Finally, the L-PRF-CS-HAP (P-C-H) composite scaffold was constructed by extrusion-based printing. The results showed that the printing ink had desirable printability and temperature sensitivity. The compressive properties of the scaffolds exhibited a trend of decline with L-PRF content increasing, but all of them could meet the strength of cancellous bone. Meanwhile, the scaffolds had high hydrophilicity, porosity, and could be degraded stably in vitro . The antibacterial properties of the scaffolds were also verified, greatly reducing the risk of infection during bone repair. It was also demonstrated that the release time of growth factor from L-PRF was significantly prolonged, and growth factor could still be detected after 35 d of sustained release. The capacity of cells to proliferate increased as the number of L-PRF components increased, indicating that L-PRF still exhibited biological activity after 3D printing.