Metal tolerant bacteria enhanced phytoextraction of lead by two accumulator ornamental species. (July 2019)

• Record Type: Journal Article
• Authors: Manzoor, Maria; Gul, Iram; Ahmed, Iftikhar; Zeeshan, Muhammad; Hashmi, Imran; Amin, Bilal Ahmad Zafar; Kallerhoff, Jean; Arshad, Muhammad
• Is Part Of: Chemosphere. Volume 227(2019)
• Page Start: 561
• Page End: 569
• Publication Date: 2019-07
• Subjects: Pb tolerant bacteria -- Phytoavailability -- PGP -- Pb phytoextraction; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2019.04.093
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Microbially enhanced availability and phytoextraction is a promising technique for phytoremediation of lead (Pb). In this study, Pb resistant strains were isolated and investigated for potential effects on plant growth and Pb phytoextraction. Incubation experiments were carried for inoculated and un–inoculated soil containing different levels of Pb. Results revealed that 20% of the isolated bacteria could tolerate Pb up to 800 mg L −1 . Five Pb resistant strains with plant growth promoting (PGP) abilities were evaluated for possible influence on water soluble Pb through soil incubation experiments and significant increase i.e. 1.85– and 1.49–folds in water soluble Pb was observed for NCCP–1848 and NCCP–1862 strains, respectively. Pot experiments indicated significantly higher uptake by Pelargonium hortorum than that by Mesembryanthemum criniflorrum at all levels of soil Pb concentrations with the highest increase (1.9–folds) in plants inoculated with NCCP–1848 followed by NCCP–1862 (1.8–folds increase) compared to the control (Pb and without bacterial strain). The strains NCCP–1848 and NCCP–1862 were identified by 16S rRNA gene sequencing as Microbacterium sp. and Klebsiella sp. The results of present study suggest that Pb resistant plant growth promoting bacteria can serve as an effective bio–inoculant through wide action spectrum for maximizing efficiency of phytoremediation system. Graphical abstract: Image 1 Highlights: Isolated bacterial strains were tolerant up to 800 mg L −1 of Pb. Bacteria within soil increased Pb phytoavailability by lowering soil pH. In vitro assays confirmed plant growth promoting (PGP) ability of bacteria. Pb resistant bacteria having PGP traits significantly promoted Pb phytoextraction.