Development of immobilized novel fungal consortium for the efficient remediation of cyanide-contaminated wastewaters. (April 2023)

• Record Type: Journal Article
• Authors: Rishi, Saloni; Kaur, Ispreet; Naseem, Mariya; Gaur, Vivek Kumar; Mishra, Sandhya; Srivastava, Suchi; Saini, Harvinder Singh; Srivastava, Pankaj Kumar
• Is Part Of: Bioresource technology. Volume 373(2023)
• Publication Date: 2023-04
• Subjects: Cyanide -- Microorganism -- Immobilized cell -- Biodegradation -- Kinetics; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2023.128750
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Novel Trichoderma strains remediated cyanide in contaminated wastewater. Co-inoculation of T. saturnisporum-T. citrinoviride degraded 99% CN in wastewater. Microbial rhodanese activity (sulfurtransferase) was dependent on GSG:GSSH ratio. First-order kinetics was the best-fitted model for CN biodegradation in the study. Abstract: Free cyanide is a hazardous pollutant released from steel industries. Environmentally-safe remediation of cyanide-contaminated wastewater is required. In this work, Pseudomonas stutzeri (ASNBRI_B12), Trichoderma longibrachiatum (ASNBRI_F9), Trichoderma saturnisporum (ASNBRI_F10) and Trichoderma citrinoviride (ASNBRI_F14) were isolated from blast-furnace wastewater and activated-sludge by enrichment culture. Elevated microbial growth, rhodanese activity (82 %) and GSSG (128 %) were observed with 20 mg-CN L −1 . Cyanide degradation > 99 % on 3rd d as evaluated through ion chromatography, followed by first-order kinetics (r 2 = 0.94–0.99). Cyanide degradation in wastewater (20 mg-CN L −1, pH 6.5) was studied in ASNBRI_F10 and ASNBRI_F14 which displayed increased biomass to 49.7 % and 21.6 % respectively. Maximum cyanide degradation of 99.9 % in 48 h was shown by an immobilized consortium of ASNBRI_F10 and ASNBRI_F14. FTIR analysis revealed that cyanide treatment alters functional groups on microbial cell walls. The novel consortium of T. saturnisporum - T. citrinoviride in the form of immobilized culture can be employed to treat cyanide-contaminated wastewater.