Triisobutyl phosphate biodegradation by enriched activated sludge consortia: Degradation mechanism and bioaugmentation potential. (1st April 2023)

• Record Type: Journal Article
• Authors: Yao, Chi; Li, Ying; Jiang, Chenxue; Li, Jing; Jing, Ke; Zhang, Suisui; Yang, Hanpei; Liu, Cheng; Zhao, Lianfang
• Is Part Of: Environmental pollution. Volume 322(2023)
• Publication Date: 2023-04-01
• Subjects: Triisobutyl phosphate -- Biotransformation pathway -- Toxic -- Microbial consortium -- Bioaugmentation potential; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2023.121027
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: This study investigated the ability of activated sludge (AS) to biodegrade triisobutyl phosphate (TiBP) after acclimation in an AS bioreactor by adding 50 mg/L TiBP. The bioreactor significantly increased the biotransformation rate of TiBP (2.15–12.7 d −1 ) over two months of acclimation. Seven transformation products (TPs) of TiBP were identified by high-resolution mass spectrometry, and hydrolysis, hydroxylation and dehydrogenation were the major biodegradation pathways of TiBP. TiBP degradation solutions at 0, 3, 7, and 10 h showed significantly toxic effects on zebrafish embryos, while the toxicity of TiBP degradation solutions at 24 h significantly decreased. Pseudomonas was inferred to be a specific bacterial population in the TiBP metabolic microbial consortium (TMMC) that degrades TiBP ( p < 0.001). When TMMC (0.5, 1, and 2 gss /L) was introduced into AS, the TiBP biotransformation rates (1.97, 2.05, and 2.26 d −1 at 1.0 mg/L TiBP, and 0.09, 0.11, and 0.83 d −1 at 30.0 mg/L TiBP) were significantly enhanced compared to the control (0.31 and 0.07 d −1 ) without TMMC inoculation. In general, this study provides new insights into the key species populations that accelerate TiBP degradation and promote the development of TiBP reduction biotechnology in WWTPs. Graphical abstract: Image 1 Highlights: Biodegradation of TiBP was significantly enhanced after acclimation. New biotransformation pathways of TiBP were proposed. Toxicity of degradation solutions was evaluated by zebrafish embryo. Pseudomonas play a major role in the degradation of TiBP. Bioaugmentation potential of TMMC for TiBP detoxification was verified.