An anti-inflammatory controlled nano drug release and pH-responsive poly lactic acid appended magnetic nanosphere for drug delivery applications. (March 2023)

• Record Type: Journal Article
• Authors: Esthar, Selvaraj; Rajesh, Jegathalaprathaban; Ayyanaar, Srinivasan; Kumar, Gujuluva Gangatharan Vinoth; Thanigaivel, Sundaram; Webster, Thomas J.; Rajagopal, Gurusamy
• Is Part Of: Materials today communications. Volume 34(2023)
• Publication Date: 2023-03
• Subjects: Poly lactic acid -- Saponin -- Magnetic microsphere -- Reactive oxygen species responsive -- Targeted drug delivery; Materials science -- Periodicals; 620.11
• DOI: 10.1016/j.mtcomm.2023.105365
• Languages: English
• ISSNs: 2352-4928

Abstract

Abstract: The drug delivery based on reactive oxygen species (ROS) sensitive saponin (SA) layered magnetic iron oxide nanoparticles (Fe3 O4 NPs) containing curcumin (CUR) encapsulated in PLA-PVA magnetic nanospheres (Fe3 O4 @SA-CUR-PLA-MMS) were synthesized and reported in this research article. The prepared nanospheres were characterized through various spectroscopic techniques such as Fourier Transform Infrared (FTIR), X-ray diffractometer (XRD), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), and Thermogravimentric Analysis (TGA) analysis. Drug release profiles demonstrated that the Fe3 O4 @SA-CUR-PLA-MMS released Curcmin (CUR) based on pH, even in the presence of ROS and hydrogen peroxide (H2 O2 ). Under slightly acidic environments of pH 5.2–7.4, the ROS-responsive polymeric nanocarriers were present in the drug release of CUR from Fe3 O4 @SA-CUR-PLA-MMS that was pH-induced. Further, Fe3 O4 @SA-CUR-PLA-MMS showed better cytotoxic properties as well as inhibited colony formation from model cancer cells including HeLa and A549 cells with IC50 values of 12.2 and 11.7 µg/mL respectively, at 24 h. Further proof of the prepared nanospheres cytotoxic activitiy towards cancer cells at 15 µg/mL for 24 h was supplied by fluorescence microscopy images obtained with the aid of AO/EB. MTT assays, fluorescence labeling microscopy, and colony formation assays suggested that the Fe3 O4 @SA-CUR-PLA-MMS might be a viable anticancer drug for numerous biomedical applications. In conclusion, the current in vitro study presents a potent nanostructured-based approach for enhanced cancer theranostics that has to be further investigated. Graphical Abstract: ga1