Biodegradation efficiency and mechanism of erythromycin degradation by Paracoccus versutus W7. (15th April 2023)

• Record Type: Journal Article
• Authors: Ren, Jianjun; Qi, Xiancheng; Zhang, Jian; Niu, Dongze; Shen, Yunpeng; Yu, Changyong; Zhi, Junqiang; Wang, Chongqing; Jiang, Xingmei; Zhang, Wenfan; Li, Chunyu
• Is Part Of: Journal of environmental management. Volume 332(2023)
• Publication Date: 2023-04-15
• Subjects: Biodegradation -- Erythromycin -- Paracoccus versutus -- Degradation pathway; Environmental policy -- Periodicals; Environmental management -- Periodicals; Environment -- Periodicals; Ecology -- Periodicals; 363.705
• DOI: 10.1016/j.jenvman.2023.117372
• Languages: English
• ISSNs: 0301-4797

Abstract

Abstract: Continuous and excessive usage of erythromycin results in serious environmental pollution and presents a health risk to humans. Biological treatment is considered as an efficient and economical method to remove it from the environment. In this study, a novel erythromycin-degrading bacterial strain, W7, isolated from sewage sludge was identified as Paracoccus versutus . Strain W7 degraded 58.5% of 50 mg/L erythromycin in 72 h under the optimal conditions of 35 °C, pH 7.0, and 0.1% sodium citrate with yeast powder in mineral salt medium. It completely eliminated erythromycin from erythromycin fermentation residue at concentrations of 100 and 300 mg/L within 36 and 60 h, respectively. Erythromycin esterase (EreA) was found to be involved in erythromycin metabolism in this strain and was expressed successfully. EreA could hydrolyze erythromycin, and its maximum activity occurred at pH 8.5 and 35 °C. Finally, six intermediates of erythromycin degraded by strain W7 were detected by high performance liquid chromatography mass spectrometry. Based on the novel intermediates and enzymes, we determined two possible pathways of erythromycin degradation by strain W7. This study broadened our understanding of the erythromycin catabolic processes of P. versutus and developed a feasible microbial strategy for removing erythromycin from erythromycin fermentation residue, wastewater, and other erythromycin-contaminated environments. Graphical abstract: Image 1 Highlights: A novel erythromycin-degrading strain Paracoccus versutus W7 was isolated. An esterase for erythromycin degradation was found in P. versutus W7. Two novel intermediates were firstly discovered from the broth. Two erythromycin degradation pathways of P. versutus W7 were illustrated. Paracoccus versutus W7 rapidly degraded erythromycin in EFR.