Influences of Ag/Ag2S core shell nanowires on the osteogenic differentiation of MC3T3-E1 cells and on the growth inhibition of methicillin-resistant Staphylococcus aureus. (March 2023)

• Record Type: Journal Article
• Authors: Oh, Hyeon Seung; Mohan, Harshavardhan; Sathya, Pavithra Muthukumar; Kim, Gitae; Ha, Ga Hyeon; Shin, Taeho
• Is Part Of: Materials today communications. Volume 34(2023)
• Publication Date: 2023-03
• Subjects: Ag -- Osteogenic differentiation -- MC3T3-E1 -- Nanowire -- Sulfidation; Materials science -- Periodicals; 620.11
• DOI: 10.1016/j.mtcomm.2022.105194
• Languages: English
• ISSNs: 2352-4928

Abstract

Abstract: Owing to their antimicrobial properties, heavy metals have been identified as promising candidates to combat bacterial infections. Silver, which is a broad-spectrum biocide, is an effective alternative to other biocides but exhibits potential cytotoxicity. Here, we synthesized Ag/Ag2 S core shell nanowires by taking advantage of structurally well-defined silver nanowires and demonstrated them as promising agents for enhancing the osteogenic differentiation of MC3T3-E1 cells and for inhibiting the growth of methicillin-resistant Staphylococcus aureus ( S. aureus ) . We examined structural, morphological, and chemical properties of the Ag/Ag2 S nanowires obtained from simple sulfidation of Ag nanowires using X-ray diffraction, scanning electron microscopy, energy dispersive X-ray spectrometry, and transmission electron microscopy. The cytotoxicity of the nanowires was examined using an MTT assay employing MC3T3-E1 cells. The sulfide shell reduced the cytotoxic nature of Ag nanowires significantly exhibiting 99% viability of cells at a concentration of 6 µg/mL. Reactive oxygen species were measured via dihydroethidium staining and cell death was quantified through Annexin V-PI staining. Overall, Ag/Ag2 S core shell nanowires assist ROS induced differentiation of osteoblasts by regulating the key marker genes runx2, osterix, and osteocalcin. A bacterial live-dead cell assay was also performed to evaluate the impact of the nanowires on the growth of S. aureus. Significant disinfection against S. aureus was demonstrated at a minimal concentration of 4 µg/mL. Our current results suggest Ag/Ag2 S nanowires as an efficient scaffolding material in both osteogenesis promotion for cells and inhibition of bacterial growth. Graphical Abstract: ga1 Highlights: Ag/Ag2S NWs showed higher biocompatibility compared to AgNWs. Ag/Ag2S NWs exhibit enhanced osteogenic differentiation in MC3T3-E1 cells. Ag/Ag2S NWs inhibit the growth of methicillin-resistant Staphylococcus aureus .