Rücktitelbild: Ligand Dilution Analysis Facilitates Aptamer Binding Characterization at the Single‐Molecule Level (Angew. Chem. 10/2023). (9th February 2023)

• Record Type: Journal Article
• Authors: Du, Yulin; Lyu, Yifan; Li, Shiquan; Ding, Ding; Chen, Jianghuai; Yang, Cai; Sun, Yang; Qu, Fengli; Xiao, Zeyu; Jiang, Jianhui; Tan, Weihong
• Is Part Of: Angewandte Chemie. Volume 135:Number 10(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2023-02-09
• Subjects: Aptamers -- Binding Site -- Dilution -- Fluorescent Probes -- Motion Analysis; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.202301456
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract : A membrane‐receptor‐analysis method termed ligand dilution analysis, which combines the concepts of single‐molecule localization and random sampling, is reported by Yifan Lyu, Jianhui Jiang, Weihong Tan et al. in their Research Article (e202215387) . Aptamer binding characterization, including receptor density calculation, single‐molecule colocalization, binding site determination, motion and fluctuation analysis, was performed with minimal probe and instrument requirements.