In vitro evaluation of copper sulfide nanoparticles decorated with folic acid/chitosan as a novel pH‐sensitive nanocarrier for the efficient controlled targeted delivery of cytarabine as an anticancer drug. (10th June 2022)

• Record Type: Journal Article
• Authors: Hamrang, Roya; Moniri, Elham; Heydarinasab, Amir; Safaeijavan, Raheleh
• Is Part Of: Biotechnology and applied biochemistry. Volume 70:Number 1(2023)
• Page Start: 330
• Page End: 343
• Publication Date: 2022-06-10
• Subjects: chitosan -- copper sulfide -- cytarabine -- drug delivery -- N‐hydroxysuccinimide -- surface modification; Biotechnology -- Periodicals; Biochemical engineering -- Periodicals; Biochemistry -- Periodicals; Biochemistry -- Periodicals; Genetic Techniques -- Periodicals; Microbiological Techniques -- Periodicals; 660.6
• DOI: 10.1002/bab.2355
• Languages: English
• ISSNs: 0885-4513

Abstract

Abstract: Nanoparticles (NPs) have gained more attention as drug delivery systems. Folic acid (FA)‐chitosan (CS) conjugates, because of their biodegradability, low toxicity, and better stability, offer a pharmaceutical drug delivery tool. The aim of this work was to fabricate CuS NPs modified by CS followed by grafting FA as a nanocarrier for the delivery of cytarabine (CYT) as an anticancer drug. In this work, CuS NPs modified by CS and FA were successfully synthesized. The structural properties of the nanocarrier were characterized by using scanning electron microscopy, Fourier transform infrared, X‐ray diffraction, thermogravimetric analysis, and Brunauer–Emmett–Teller. The adsorption mechanism of CYT by adsorption isotherms, kinetics, and thermodynamics was deliberated and modeled. The in vitro CYT release behavior for the nanocarrier was 99% and 61% at pH 5.6 and 7.4, respectively. The adsorption behavior of CYT by CuS NPs –CS–FA was well explored by pseudo–second‐order kinetic and Langmuir isotherm models by the coefficient of determination ( R 2 > 0.99). Thermodynamic results showed that the uptake of CYT by CuS NPs‐CS‐FA was endothermic and spontaneous. The experimental results showed that CYT/CuS NPs ‐CS‐FA can be proposed as an efficient nanocarrier for the targeted delivery of anticancer drugs. Abstract : This work aimed to synthesis copper sulphide nanoparticles (CuS NPs) modified with chitosan (CS) followed by conjugated with folic acid (FA) to facilitate the targeted release of cytarabine (CYT) anti‐cancer drug, and this pHsensitive nanocarrier was named as CYT/CuS NPs‐CS‐FA.