A convenient separation strategy for fungal anthraquinones by centrifugal partition chromatography. Issue 5 (19th January 2022)

• Record Type: Journal Article
• Authors: Hammerle, Fabian; Zwerger, Michael; Höck, Anja; Ganzera, Markus; Peintner, Ursula; Siewert, Bianka
• Is Part Of: Journal of separation science. Volume 45:Issue 5(2022)
• Page Start: 1031
• Page End: 1041
• Publication Date: 2022-01-19
• Subjects: anthraquinone glycosides -- anthraquinones -- basidiomycetes -- centrifugal partition chromatography -- cortinarius fruiting bodies; Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.202100869
• Languages: English
• ISSNs: 1615-9306

Abstract

Abstract: As recently shown, some fungal pigments exhibit significant photoactivity turning them into promising agents for the photodynamic treatment of microbial infections or malignant diseases. In the present study, a separation strategy for fungal anthraquinones was developed based on centrifugal partition chromatography. A suitable method was explored employing a methanolic extract of the fruiting bodies of Cortinarius sanguineus (Agaricales, Basidiomycota). An excellent fractionation was achieved using a biphasic solvent system comprising chloroform/ethyl acetate/methanol/water/acetic acid (3:1:3:2:1, v/v/v/v/v) operating in ascending mode. Experiments on an analytical scale with extracts of closely related Cortinarius species exhibited broad applicability of the devised system. Up to six pigments could be purified directly from the crude extract. Preparative‐scale fractionation of the methanol extracts of C. malicorius and C. sanguineus demonstrated that up‐scaling was possible without compromising selectivity.