G‐Quadruplex Binding of an NIR Emitting Osmium Polypyridyl Probe Revealed by Solution NMR and Time‐Resolved Infrared Studies. Issue 11 (12th January 2023)

• Record Type: Journal Article
• Authors: Peterková, Kateřina; Stitch, Mark; Boota, Rayhaan Z.; Scattergood, Paul A.; Elliott, Paul I. P.; Towrie, Michael; Podbevšek, Peter; Plavec, Janez; Quinn, Susan J.
• Is Part Of: Chemistry. Volume 29:Issue 11(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2023-01-12
• Subjects: G-quadruplex -- luminescent enhancement -- NMR -- osmium polypyridyl -- spectroscopy -- TRIR; Chemistry -- Periodicals; 540
• DOI: 10.1002/chem.202203250
• Languages: English
• ISSNs: 0947-6539

Abstract

Abstract: G‐quadruplexes are emerging targets in cancer research and understanding how diagnostic probes bind to DNA G‐quadruplexes in solution is critical to the development of new molecular tools. In this study the binding of an enantiopure NIR emitting [Os(TAP)2 (dppz)] 2+ complex to different G‐quadruplex structures formed by human telomer (hTel) and cMYC sequences in solution is reported. The combination of NMR and time‐resolved infrared spectroscopic techniques reveals the sensitivity of the emission response to subtle changes in the binding environment of the complex. Similar behaviour is also observed for the related complex [Os(TAP)2 (dppp2)] 2+ upon quadruplex binding. Abstract : Luminescent enhancement of an enantiopure Os polypyridyl probe containing an extended dipyrido[3, 2‐a:2′, 3′‐c]phenazine (dppz) ligand in the presence of human telomer (hTel) and cMYC guanine quadruplexes (G4 s) is reported. NMR studies indicate the role of π‐π stacking interactions at the 5'‐quartet end, which is supported by time‐resolved infrared measurements. Importantly, the combined measurements indicate the sensitivity of luminescent enhancement to the particular orientation at the 5'‐quartet binding site.