Purification and characterization of a chitinase from Aeromonas media CZW001 as a biocatalyst for producing chitinpentaose and chitinhexaose. (12th June 2022)

• Record Type: Journal Article
• Authors: Ding, Zhiwen; Li, Tian; Chen, Meng; Fang, Yaowei; Hou, Xiaoyue; Yang, Gaung; Lu, Jing; Ye, Qinwen; Zhu, Rongjun; He, Fuxiang; Xia, Mengjie; Liu, Shu
• Is Part Of: Biotechnology and applied biochemistry. Volume 70:Number 1(2023)
• Page Start: 281
• Page End: 289
• Publication Date: 2022-06-12
• Subjects: AmChi -- antifungal activity -- characterization -- chitinase; Biotechnology -- Periodicals; Biochemical engineering -- Periodicals; Biochemistry -- Periodicals; Biochemistry -- Periodicals; Genetic Techniques -- Periodicals; Microbiological Techniques -- Periodicals; 660.6
• DOI: 10.1002/bab.2351
• Languages: English
• ISSNs: 0885-4513

Abstract

Abstract: Developing chitinase suitable for the bioconversion of chitin to chitin oligosaccharides has attracted significant attention due to its benefits in environmental protection. In this study, chitinase from Aeromonas media CZW001 ( Am Chi) was purified and characterized. The molecular weight of Am Chi was approximately 40 kDa. Am Chi exhibited maximum catalytic activity at pH 8.0 with an optimum temperature of 55°C and showed broad stability between 15 and 65°C and between pH 5.0 and 9.0. Am Chi was activated by Mg 2+, Na +, and K + and inhibited by Hg +, Co 2+, Fe 2+, Ca 2+, Ag +, Zn 2+, and EDTA. The main products of Am Chi on colloidal chitin were chitinhexaose and chitinpentaose. Am Chi had better substrate specificity for powdered chitin than colloidal chitin and had a higher catalytic efficiency toward (GlcNAc)5 than colloidal chitin. Am Chi inhibited fungal growth in a dose‐dependent manner. These results suggest that Am Chi could be used for the enzymatic degradation of chitin to produce chitinhexaose and chitinpentaose, which have several industrial applications. Abstract : Separation, purification and degradation products of AmChi