Development and validation of a mass spectrometry based analytical method to quantify the ratios in hemagglutinin trimers in quadrivalent influenza nanoparticle vaccine – FluMos-v1. Issue 7 (1st February 2023)

• Record Type: Journal Article
• Authors: Shajahan, Asif; Cai, Cindy X.; Wolff, Jeremy; Yang, R. Sylvie; Ivleva, Vera B.; Gowetski, Daniel B.; Gall, Jason G. D.; Lei, Q. Paula
• Is Part Of: Analytical methods. Volume 15:Issue 7(2023)
• Page Start: 896
• Page End: 900
• Publication Date: 2023-02-01
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/d2ay01890j
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : A strategy for the evaluation of different HAT (hemagglutinin variants) ratios on the FluMos-v1 mosaic nanoparticle influenza vaccine by LC-MS/MS-based PRM. Abstract : A quadrivalent influenza nanoparticle vaccine (FluMos-v1) offers long-lasting protection against multiple influenza virus strains and is composed of four strains of hemagglutinin trimer (HAT) assembled around a pentamer core. Here we report an LC-MS/MS analytical development and validation method to measure the percentage of each HAT component in FluMos-v1.