Development of new Ti50Zr25Nb20Cu5–xAgx high-entropy alloys with excellent antibacterial property, osteo-conductivity and biocompatibility in vitro and in vivo. (1st April 2023)

• Record Type: Journal Article
• Authors: Yu, Bingxiao; Juma, Talante; Wang, Hao; Bao, Xiaotong; Cao, Xiangyu; Wang, Zhiwen; Wang, Rui; Yang, Xin; Ning, Taiguo; Liang, Guanghua; Cao, Yongping; Zhang, Tao; Guan, Zhenpeng
• Is Part Of: Journal of materials science & technology. Volume 141(2023)
• Page Start: 209
• Page End: 220
• Publication Date: 2023-04-01
• Subjects: High-entropy alloys -- Implant infection -- Metal anti-bacteria -- Biofilm -- Osteo-conductivity -- Biocompatibility; HEAs High-entropy alloys -- ALP Alkaline phosphatase -- SD Sprague Dawley -- HFCL Human fibroblasts -- DMEM Dulbecco's modified Eagle medium -- RGR Relative growth rate -- BCA Bicinchoninic acid -- PCR Polymerase chain reaction -- MC3T3-E1 Murine osteoblast -- WBC White blood cells -- NEUT Neutrophils -- PCT Procalcitonin -- CRP C-reactive protein -- ESR Erythrocyte sedimentation rate -- IL-1 Interleukin 1 -- SEM Scanning electron microscopy -- HPF High power field -- HGB Hemoglobin -- Cre Creatinine -- ALT Alanine aminotransferase -- OD Optical density -- HA Hydroxyapatite -- PHF Per high-power field; Metals -- Periodicals; Materials science -- Periodicals; Materials science; Metals; Periodicals; 620.1105
• DOI: 10.1016/j.jmst.2022.07.064
• Languages: English
• ISSNs: 1005-0302

Abstract

Highlights: New Zr–Ti–Nb–Cu–Ag high-entropy alloys(HEAs), especially HEACu2.5 Ag2.5 showed better antibacterial ability against Staphylococcus aureus than Ti–6Al–4V. Excellent osteo-conductivity of HEAs was observed in vitro . HEACu2.5 Ag2.5 can prevent serious bacterial infections peri-implants, with a lower PCT in infection models. Abstract: A new series of medical antibacterial high-entropy alloys (HEAs) with composition Ti50 Zr25 Nb20 Cu5– x Ag x ( x = 0 at.%, 1 at.%, and 2.5 at.%) were developed by arc-melting the mixture of pure elements under a high-purity argon atmosphere. The cytotoxicity, osteo-conductivity, and antibacterial properties of these HEAs were systematically investigated in vitro, along with their in vivo biocompatibility and antibacterial properties. These HEAs, especially Ti50 Zr25 Nb20 Cu2.5 Ag2.5, showed better antibacterial ability against Staphylococcus aureus than Ti–6Al–4V, which has a nearly 99% antibacterial rate in vitro . Moreover, ion release and cytotoxicity tests showed that these HEAs had excellent biosafety, similar to or better than that of Ti–6Al–4V. Osteoblasts were used to evaluate the osteogenic activity of these HEAs, and good osteo-conductivity was observed in vitro . In a Sprague-Dawley rat infection model, Ti50 Zr25 Nb20 Cu2.5 Ag2.5 showed remarkable antibacterial properties compared to Ti–6Al–4V. The in vivo biocompatibility results showed that Ti50 Zr25 Nb20 Cu2.5 Ag2.5 did not increase biotoxicity compared to Ti–6Al–4V. The excellent biocompatibility and antibacterial properties of the Ti–Zr–Nb–Cu–Ag HEAs are expected to enable the prevention of periprosthetic/peri-implant infections in the future.