OsLDDT1, encoding a transmembrane structural DUF726 family protein, is essential for tapetum degradation and pollen formation in rice. (April 2023)

• Record Type: Journal Article
• Authors: Sun, Zhihao; Liu, Keke; Chen, Chi; Chen, Daibo; Peng, Zequn; Zhou, Ran; Liu, Ling; He, Dengmei; Duan, Wenjing; Chen, Hongmei; Huang, Chenbo; Ruan, Zheyan; Zhang, Yingxin; Cao, Liyong; Zhan, Xiaodeng; Cheng, Shihua; Sun, Lianping
• Is Part Of: Plant science. Volume 329(2023)
• Publication Date: 2023-04
• Subjects: Rice -- Male sterility -- OsLDDT1 -- Fatty acid synthesis -- Transmembrane structural protein; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2023.111596
• Languages: English
• ISSNs: 0168-9452

Abstract

Abstract: Formation of the pollen wall, which is mainly composed of lipid substances secreted by tapetal cells, is important to ensure pollen development in rice. Although several regulatory factors related to lipid biosynthesis during pollen wall formation have been identified in rice, the molecular mechanisms controlling lipid biosynthesis are unclear. In this study, we isolated the male-sterile rice mutant oslddt1 ( leaked and delayed degraded tapetum 1 ). oslddt1 plants show complete pollen abortion resulting from delayed degradation of the tapetum and blocked formation of Ubisch bodies and pollen walls. OsLDDT1 ( LOC_Os03g02170 ) encodes a DUF726 containing protein of unknown function with highly conserved transmembrane and α/β Hydrolase domains. OsLDDT1 localizes to the endoplasmic reticulum and the gene is highly expressed in rice panicles. Genes involved in regulating fatty acid synthesis and formation of sporopollenin and pollen exine during anther development showed significantly different expression patterns in oslddt1 plants. Interestingly, the wax and cutin contents in mature oslddt1–1 anthers were decreased by 74.07 % and 72.22 % compared to WT, indicating that OsLDDT1 is involved in fatty acid synthesis and affects formation of the anther epidermis. Our results provide as deeper understanding of the role of OsLDDT1 in regulating male sterility and also provide materials for hybrid rice breeding. Highlights: OsLDDT1 encodes a DUF726 family protein, which regulates tapetum PCD and pollen formation. Alternative splicing mutation of OsLDDT1 cause delayed tapetum PCD and pollen degradation. OsLDDT1 affects fatty acid synthesis by patterning of Ubisch bodies and pollen exine