Spirometra mansoni sparganosis identified by metagenomic next-generation sequencing: a case report. (March 2023)

• Record Type: Journal Article
• Authors: Hu, Diefei; Jin, Wenxiang; Ding, Huarong; Pang, Yu; Ma, Shasha; Yang, Min; Wu, Shuwen; Jiang, Muliang; Pang, Lingling; Luo, Shuming; Ning, Qiuyue
• Is Part Of: International journal of infectious diseases. Volume 128(2023)
• Page Start: 128
• Page End: 131
• Publication Date: 2023-03
• Subjects: Metagenomic next-generation sequencing -- Spirometra mansoni -- Infection; Communicable diseases -- Periodicals; Communicable Diseases -- Periodicals; Communicable diseases; Periodicals; Electronic journals; 616.9
• DOI: 10.1016/j.ijid.2022.12.038
• Languages: English
• ISSNs: 1201-9712

Abstract

Highlights: A 30-year-old male was infected with the larva of Spirometra mansoni . Spirometra mansoni sparganosis was identified by metagenomic next-generation sequencing. Metagenomic next-generation sequencing can provide a reliable method in parasite species identification. Abstract : A 30-year-old male patient had a cyst on the left hip and progressive enlargement for more than 2 months. Combined blood tests, magnetic resonance imaging, and pathology findings, cysticercosis infection was suspected. However, the treatment for cysticercosis was ineffective. We conducted a metagenomic next-generation sequencing (mNGS) analysis on the formalin-fixed, paraffin-embedded specimen of the patient's surgically excised tissue, and the results suggested Spirometra mansoni, mNGS was further confirmed by polymerase chain reaction and phylogenetic analysis of cytochrome c oxidase subunit 1 ( cox1) gene. Based on these results, we found that mNGS provided a better method of diagnosing parasitic infections.