Evaluation of Phytochemical Contents, Antimicrobial, and antioxidant Potential of Haloxylon Griffithii Collected From Northern Region of Balochistan, Pakistan. Issue 1 (16th March 2023)

• Record Type: Journal Article
• Authors: Asif, Rizwan; Ali, Muhammad; Mazhar, Modasrah; Mustafa, Madiha; Yasmin, Riffat
• Is Part Of: Dose-response. Volume 21:Issue 1(2023)
• Publication Date: 2023-03-16
• Subjects: H griffithii -- GC/MS -- phytochemical analysis -- antioxidant -- antimicrobial; Dose-response relationship (Biochemistry) -- Periodicals; Drugs -- Dose-response relationship -- Periodicals; Drugs -- Physiological effect -- Periodicals; Hormesis -- Periodicals; Dose-Response Relationship, Drug -- Periodicals; Dose-response relationship (Biochemistry); Drugs -- Dose-response relationship; Drugs -- Physiological effect; Periodicals; 571.634
• DOI: 10.1177/15593258231163389
• Languages: English
• ISSNs: 1559-3258

Abstract

Background: The therapeutic potential of Haloxylon griffithii found in northern region of Balochistan, so far has been neglected. Purpose: The current study was aimed to assess the phytochemicals and pharmacological potential of fractions isolated from H griffithii . Research Design: During phytochemicals analysis of H griffithii using GC/MS showed various bioactive compounds like alkaloids, flavonoids, terpenoids, tannins, saponins, and carboxylic acids. In vitro antioxidant activity of H griffithii was determined by 2, 2'- diphenyl-1-picrylhydrazyl (DPPH)assay. Disc diffusion method was used to evaluate the antimicrobial activity. Results: The quantitative analysis of ethyl acetate showed highest total flavonoid contents (1.19 ± .05) while ethanol with lowest value (.52 ± .01). The total phenolic contents in ethyle acetate was 1.50 ± .42, whereas ethanol showed lowest value (.77 ± .02). Ethanol exhibited excellent (88.68 ± 3.0) free radical scavenging potential measured by 1, 1-diphenyl-2-picryl-hydrazyl radical scavenging assay. For antimicrobial activity, different bacterial and fungal strains like B subtilis, S aureus, E coli, S typhi, C albicans, and A. niger were selected . The essential oil showed maximum % inhibition diameter (9 mm) against B. Subtillus and (5 mm) against C albicans, respectively . The ethyl acetate presented % inhibition diameter (9 mm) against S aureus and (6 mm) against A niger. Anti-urease activity also showed positive response. Conclusions: The presence of high (%) bioactive compounds with great therapeutic potential suggest that H griffithii can be used as natural alternative of synthetic drugs without side effects.