Retinex-qDPC: Automatic background-rectified quantitative differential phase contrast imaging. (March 2023)

• Record Type: Journal Article
• Authors: Zhang, Shuhe; Peng, Tao; Ke, Zeyu; Yang, Han; Berendschot, Tos T.J.M.; Zhou, Jinhua
• Is Part Of: Computer methods and programs in biomedicine. Volume 230(2023)
• Publication Date: 2023-03
• Subjects: Retinex theory -- Quantitative phase retrieval -- Differential phase contrast imaging -- Background correction -- Deconvolution; Medicine -- Computer programs -- Periodicals; Biology -- Computer programs -- Periodicals; Computers -- Periodicals; Medicine -- Periodicals; Médecine -- Logiciels -- Périodiques; Biologie -- Logiciels -- Périodiques; Biology -- Computer programs; Medicine -- Computer programs; Periodicals; Electronic journals; 610.28
• DOI: 10.1016/j.cmpb.2022.107327
• Languages: English
• ISSNs: 0169-2607

Abstract

Highlighs: We proposed a novel reconstruction algorithm, termed Retinex-qDPC, for quantitative differential phase contrast imaging based on Retinex theory. The Retinex-qDPC tackles the background mismatch problem, and significantly increases the phase reconstruction quality and experimental robustness. Both L2 -Retinex and L1 -Retinex are studied in our research, both outperform other state-of-the-art qDPC reconstruction algorithm. While the L1 -Retinex performed better than L2 -Retinex in our case. The Retinex-qDPC doesn't require any modification to the optical system will benefit for the whole DPC family. Abstract: Background and objective: The quality of quantitative differential phase contrast reconstruction (qDPC) can be severely degenerated by the mismatch of the background of two oblique illuminated images, yielding problematic phase recovery results. These background mismatches may result from illumination patterns, inhomogeneous media distribution, or other defocusing layers. In previous reports, the background is manually calibrated which is time-consuming, and unstable, since new calibrations are needed if any modification to the optical system was made. It is also impossible to calibrate the background from the defocusing layers, or for high dynamic observation as the background changes over time. The background mismatch reduces the experimental robustness of qDPC and largely limits its applications. To tackle the mismatch of background and increases the experimental robustness, we propose the Retinex-qDPC. Methods: In Retinex-qDPC, we replace the data fidelity term of the previous cost function for qDPC inverse problem, by the images' edge features yielding L2 -Retinex-qDPC and L1 -Retinex-qDPC for high background-robustness qDPC reconstruction. The split Bregman method is used to solve the L1 -Retinex DPC. We compare both Retinex-qDPC models against state-of-the-art DPC reconstruction algorithms including total-variation regularized qDPC, and isotropic-qDPC using both simulated and experimental data. Results: Retinex qDPC can significantly improve the phase recovery quality by suppressing the impact of mismatch background. Within, the L1 -Retinex-qDPC is better than L2 -Retinex and other state-of-the-art qDPC algorithms. Conclusions: The Retinex-qDPC increases the experimental robustness against background illumination without any modification of the optical system, which will benefit all qDPC applications.