Paired associative stimulation on the soleus H-Reflex using motor point and peripheral nerve stimulation. (16th February 2023)

• Record Type: Journal Article
• Authors: Fok, Kai Lon; Kaneko, Naotsugu; Tajali, Shirin; Masani, Kei
• Is Part Of: Neuroscience letters. Volume 797(2023)
• Publication Date: 2023-02-16
• Subjects: H-reflex -- Motor point stimulation -- Peripheral nerve stimulation -- Paired associative stimulation; PNS peripheral nerve stimulation -- MPS motor point stimulation -- PAS paired associative stimulation; Neurology -- Periodicals; Neurology -- Periodicals; Research -- Periodicals; Neurologie -- Périodiques; Neuroanatomie -- Périodiques; Neuropharmacologie -- Périodiques; Neurophysiologie -- Périodiques; Neurology; Periodicals; Electronic journals; 617.48
• DOI: 10.1016/j.neulet.2023.137070
• Languages: English
• ISSNs: 0304-3940

Abstract

Highlights: Paired associative stimulation has been used to modulate corticospinal excitability. Motor point stimulation can activate the α motoneuron cell body antidromically. We aimed to suppress the spinal H-reflex using paired associative stimulation. Motor and peripheral nerve stimulation targeted the Ia-α motoneuron synapse. The spinal H-reflex was unchanged after paired associative stimulation. Abstract: Paired associative stimulation (PAS) has been shown to modulate the corticospinal excitability via spike timing dependent plasticity (STDP). In this study, we aimed to suppress the spinal H-Reflex using PAS. We paired two stimulation modalities, i.e., peripheral nerve stimulation (PNS) and motor point stimulation (MPS). We used PNS to dominantly activate the Ia sensory axon, and we used MPS to dominantly activate the α-motoneuron cell body antidromically. Thus, we applied both PNS and MPS such that the α-motoneuron cell body was activated 5 ms before the activation of the Ia sensory axon ending at the Ia-α motoneuron synapse. If the spinal reflexes can be modulated by STDP, and a combination of MPS and PNS is timed appropriately, then the H-Reflex amplitude will decrease while no change in H-Reflex amplitude is expected for MPS or PNS only. To test this hypothesis, six young healthy participants (5M/1F: 26.8 ± 4.1 yrs) received one of the three following conditions on days separated by at least 24 hr: 1) PAS, 2) MPS only or 3) PNS only. The H-Reflex and M-wave recruitment curves of the soleus were measured immediately prior to, immediately after, 30 min and 60 min after the intervention. The normalized H-Reflex amplitudes were then compared across conditions and times using a two-way ANOVA (3 conditions × 4 times). No main effects of condition or time, or interaction effect were found. These results suggest that relying solely on STDP may be insufficient to inhibit the soleus H-Reflex.