Cellular Detection of a Mitochondria Targeted Brominated Vinyl Triphenylamine Optical Probe (TP−Br) by X‐Ray Fluorescence Microscopy. Issue 15 (23rd February 2022)

• Record Type: Journal Article
• Authors: Nagarajan, Sounderya; Poyer, Florent; Fourmois, Laura; Naud‐Martin, Delphine; Medjoubi, Kadda; Somogyi, Andrea; Schanne, Gabrielle; Henry, Lucas; Delsuc, Nicolas; Policar, Clotilde; Bertrand, Helene C.; Mahuteau‐Betzer, Florence
• Is Part Of: Chemistry. Volume 28:Issue 15(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-02-23
• Subjects: bimodal probes -- fluorescence microscopy -- mitochondria -- organic chemistry -- X-Ray fluorescence; Chemistry -- Periodicals; 540
• DOI: 10.1002/chem.202104424
• Languages: English
• ISSNs: 0947-6539

Abstract

Abstract: Triphenylamine (TP) derivatives such as two‐branch cationic vinylbenzimidazolium triphenylamine TP−2Bzim are promising turn‐on fluorescent probes suitable for two‐photon imaging, labelling mitochondria in live cells. Here, we designed two TP−2Bzim derivatives as bimodal probes suitable for X‐ray fluorescence imaging. The conjugation of the TP core with a rhenium tricarbonyl moiety in the TP−RePyta probe altered the localisation in live cells from mitochondria to lysosomes. The introduction of bromine on the TP core generated the TP−Br probe retaining good photophysical properties and mitochondria labelling in live cells. The influence of calcium channels in the uptake of TP−Br was studied. Synchrotron Radiation X‐ray Fluorescence (SXRF) imaging of bromine enabled the detection of TP−Br and suggested a negligible presence of the probe in an unbound state in the incubated cells, a crucial point in the development of these probes. This study paves the way towards the development of TP probes as specific organelle stainers suitable for SXRF imaging. Abstract : We designed and synthesized a new bimodal vinyltriphenylamine probe. TP−Br is an off‐on fluorescent probe that targets mitochondria thanks to its lipophilic cationic structure. The probe was imaged by two modalities in cells: by one‐photon or two‐photon excitation fluorescence microscopy and by Scanning Synchrotron Radiation X‐ray Fluorescence.