Dark microbial CO2 fixation in temperate forest soils increases with CO2 concentration. (31st December 2019)

• Record Type: Journal Article
• Authors: Spohn, Marie; Müller, Karolin; Höschen, Carmen; Mueller, Carsten W.; Marhan, Sven
• Is Part Of: Global change biology. Volume 26:Number 3(2020)
• Page Start: 1926
• Page End: 1935
• Publication Date: 2019-12-31
• Subjects: anaplerotic reactions -- carbon cycle -- chemoautotrophic bacteria -- CO2 concentration -- dark microbial CO2 fixation -- fungal–bacterial interactions -- microbial carbon pump -- microbial soil carbon processing -- soil organic matter formation; Climatic changes -- Environmental aspects -- Periodicals; Troposphere -- Environmental aspects -- Periodicals; Biodiversity conservation -- Periodicals; Eutrophication -- Periodicals; 551.5
• DOI: 10.1111/gcb.14937
• Languages: English
• ISSNs: 1354-1013

Abstract

Abstract: Dark, that is, nonphototrophic, microbial CO2 fixation occurs in a large range of soils. However, it is still not known whether dark microbial CO2 fixation substantially contributes to the C balance of soils and what factors control this process. Therefore, the objective of this study was to quantitate dark microbial CO2 fixation in temperate forest soils, to determine the relationship between the soil CO2 concentration and dark microbial CO2 fixation, and to estimate the relative contribution of different microbial groups to dark CO2 fixation. For this purpose, we conducted a 13 C‐CO2 labeling experiment. We found that the rates of dark microbial CO2 fixation were positively correlated with the CO2 concentration in all soils. Dark microbial CO2 fixation amounted to up to 320 µg C kg −1 soil day −1 in the Ah horizon. The fixation rates were 2.8–8.9 times higher in the Ah horizon than in the Bw1 horizon. Although the rates of dark microbial fixation were small compared to the respiration rate (1.2%–3.9% of the respiration rate), our findings suggest that organic matter formed by microorganisms from CO2 contributes to the soil organic matter pool, especially given that microbial detritus is more stable in soil than plant detritus. Phospholipid fatty acid analyses indicated that CO2 was mostly fixed by gram‐positive bacteria, and not by fungi. In conclusion, our study shows that the dark microbial CO2 fixation rate in temperate forest soils increases in periods of high CO2 concentrations, that dark microbial CO2 fixation is mostly accomplished by gram‐positive bacteria, and that dark microbial CO2 fixation contributes to the formation of soil organic matter. Abstract : The contribution of dark, that is, nonphototrophic, microbial CO2 fixation in soil to the carbon cycle is still not well understood. We found that the rates of dark microbial CO2 fixation in temperate forest soils were positively correlated with the CO2 concentration, indicating that more CO2 is fixed in periods of high soil CO2 concentrations and in microsites with high CO2 concentration. In addition, our findings suggest that organic matter formed by microorganisms from CO2 contributes to the soil organic matter pool and that dark CO2 fixation in temperate forest soils is mostly accomplished by gram‐positive bacteria and not by fungi.