Characterization of the contractile P2Y14 receptor in mouse coronary and cerebral arteries. Issue 17 (6th June 2014)

• Record Type: Journal Article
• Authors: Haanes, Kristian Agmund; Edvinsson, Lars
• Is Part Of: FEBS letters. Volume 588:Issue 17(2014)
• Page Start: 2936
• Page End: 2943
• Publication Date: 2014-06-06
• Subjects: Purinergic receptors -- P2Y14 -- Coronary artery -- Basilar artery -- Receptor upregulation -- P2Y2 KO; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2014.05.044
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Extracellular UDP‐glucose can activate the purinergic P2Y14 receptor. The aim of the present study was to examine the physiological importance of P2Y14 receptors in the vasculature. The data presented herein show that UDP‐glucose causes contraction in mouse coronary and basilar arteries. The EC50 values and immunohistochemistry illustrated the strongest P2Y14 receptor expression in the basilar artery. In the presence of pertussis toxin, UDP‐glucose inhibited contraction in coronary arteries and in the basilar artery it surprisingly caused relaxation. After organ culture of the coronary artery, the EC50 value decreased and an increased staining for the P2Y14 receptor was observed, showing receptor plasticity.