RNA interference‐mediated silencing of vATPase subunits A and E affect survival and development of the 28‐spotted ladybeetle, Henosepilachna vigintioctopunctata. (9th January 2021)

• Record Type: Journal Article
• Authors: Guo, Wei; Guo, Mujuan; Yang, Chunxiao; Liu, Zhuoqi; Chen, Shimin; Lü, Jing; Qiu, Baoli; Zhang, Youjun; Zhou, Xuguo; Pan, Huipeng
• Is Part Of: Insect science. Volume 28:Number 6(2021)
• Page Start: 1664
• Page End: 1676
• Publication Date: 2021-01-09
• Subjects: dietary RNAi -- Henosepilachna vigintioctopunctata -- mortality -- nontarget effects -- Propylaea japonica -- vATPase; Insects -- Periodicals; Entomology -- Periodicals; 595.705
• DOI: 10.1111/1744-7917.12899
• Languages: English
• ISSNs: 1672-9609

Abstract

Abstract: RNA interference (RNAi) has emerged as a powerful tool for developing novel management strategies for controlling insect pests. The 28‐spotted ladybeetle, Henosepilachna vigintioctopunctata is one of the most important pests attacking solanaceous plants in Asia. In this study, the potential of dietary RNAi to manage H. vigintioctopunctata was investigated using both in vitro synthesized and bacterially expressed double‐stranded RNAs (dsRNAs) of HvvATPase A and HvvATPase E . The expression levels of HvvATPase A and HvvATPase E were higher in Malpighian tubules than in other tissue types. The silencing of HvvATPase A and HvvATPase E led to significant mortality in H. vigintioctopunctata larvae. In addition, the ingestion of HvvATPase A and HvvATPase E significantly deterred feeding behavior and subsequently arrested the development of H. vigintioctopunctata . Notably, the bacterially expressed dsRNAs consistently caused higher mortality in larvae and adults. Finally, the nontarget effects of the dsRNAs of H. vigintioctopunctata on the predatory ladybeetle Propylaea japonica were evaluated. P. japonica 1st instar larvae were administered vATPase A and vATPase E dsRNAs from H. vigintioctopunctata and P. japonica under the worst‐case scenario, in which ds GFP served as negative control. There were significant effects of ds HvvATPase A on P. japonica at the transcriptional level but not at the organismal level, whereas ds HvvATPase E did not effect P. japonica at either the transcriptional or the organismal level. Collectively, the results of the study suggest that HvvATPase A and HvvATPase E can act as novel molecular targets for the control of H. vigintioctopunctata . Abstract : Silencing of in vitro synthesized HvvATPase A and HvvATPase E led to significant mortality in Henosepilachna vigintioctopunctata larvae. Ingestion of HvvATPase A and HvvATPase E significantly deterred the feeding behavior and, subsequently, arrested the development of H. vigintioctopunctata . Bacterially expressed double‐stranded RNAs (dsRNAs) consistently caused a higher mortality in both larvae and adults. dsHvvATPase A had impact on Propylaea japonica at transcriptional level but not at the organismal level, whereas dsHvvATPase E had no impact on P. japonica at either transcriptional or organismal levels. HvvATPase A and HvvATPase E can serve as novel molecular targets for controlling H. vigintioctopunctata .