Intravascular Radiocontrast Iodixanol Increases Permeability of Proximal Tubule Epithelium: A Possible Mechanism of Contrast-Induced Nephropathy. (November 2013)

• Record Type: Journal Article
• Authors: Yao, Luyu; Kolluru, Gopi K.; Kevil, Christopher G.; Zhang, Wayne W.
• Is Part Of: Vascular & endovascular surgery. Volume 47:Number 8(2013)
• Page Start: 632
• Page End: 638
• Publication Date: 2013-11
• Subjects: iodixanol -- contrast-induced nephropathy -- proximal tubule epithelium -- permeability; Blood-vessels -- Surgery -- Periodicals; Blood-vessels -- Endoscopic surgery -- Periodicals; Vascular Surgical Procedures -- Periodicals; Angioplasty -- Periodicals; Surgical Procedures, Minimally Invasive -- Periodicals; Vascular Diseases -- surgery -- Periodicals; Vaisseaux sanguins -- Chirurgie -- Périodiques; Vaisseaux sanguins -- Chirurgie endoscopique -- Périodiques; 617.41
• DOI: 10.1177/1538574413503560
• Languages: English
• ISSNs: 1538-5744

Abstract

Purpose: To investigate the effect of Iodixanol on kidney proximal tubular cell line human kidney 2 (HK-2). Methods: The HK-2 cells were treated with Iodixanol. A Terminal deoxynucleotidyl transferase dUTP nick-end labeling ( TUNEL ) assay was used to evaluate apoptosis. Cell viability was measured by proliferation assay kit. Cell permeability changes were assessed by transwell assay and intercellular gaps measurement. Expression of claudin-2 was assessed by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) and Western blot. Results: Iodixanol reduced tubule cell viability ( P < .01) but did not cause apoptosis. The intercellular gap formation ( P < .01) and transwell ( P < .05) assays revealed that cell permeability significantly increased after Iodixanol treatment of monolayer cells. Western blot and qRT-PCR showed significant upregulation of claudin-2 protein ( P < .05) and messenger RNA expression ( P < .01). Conclusions: Our in vitro data do not support the hypothesis that direct kidney cell death from Iodixanol is a major mechanism of contrast-induced nephropathy (CIN). However, increased permeability of proximal tubule epithelium caused by Iodixanol may play an important role in CIN.