An Outbreak of Human Coronavirus OC43 Infection and Serological Cross-Reactivity with SARS Coronavirus. Issue 6 (1st December 2006)

• Record Type: Journal Article
• Authors: Patrick, David M; Petric, Martin; Skowronski, Danuta M; Guasparini, Roland; Booth, Timothy F; Krajden, Mel; McGeer, Patrick; Bastien, Nathalie; Gustafson, Larry; Dubord, Janet; MacDonald, Diane; David, Samara T; Srour, Leila F; Parker, Robert; Andonov, Anton; Isaac-Renton, Judith; Loewen, Nadine; McNabb, Gail; McNabb, Alan; Goh, Swee-Han; Henwick, Scott; Astell, Caroline; Guo, Jian Ping; Drebot, Michael; Tellier, Raymond; Plummer, Francis; Brunham, Robert C
• Is Part Of: Canadian journal of infectious diseases & medical microbiology =. Volume 17:Issue 6(2006)
• Page Start: 330
• Page End: 336
• Publication Date: 2006-12-01
• Subjects: Coronavirus -- Human coronavirus OC43 -- Outbreak -- Respiratory infection -- SARS-CoV -- SARS coronavirus -- Severe acute respiratory syndrome; Communicable diseases -- Periodicals; Infection -- Periodicals; Communicable diseases; Infection; Communicable Diseases; Communicable Disease Control; Electronic journals; Periodicals; Fulltext; Internet Resources; Periodicals; 616.9
• DOI: 10.1155/2006/152612
• Languages: English
• ISSNs: 1712-9532

Abstract

Abstract : BACKGROUND: In summer 2003, a respiratory outbreak was investigated in British Columbia, during which nucleic acid tests and serology unexpectedly indicated reactivity for severe acute respiratory syndrome coronavirus (SARS-CoV). METHODS: Cases at a care facility were epidemiologically characterized and sequentially investigated for conventional agents of respiratory infection, SARS-CoV and other human CoVs. Serological cross-reactivity between SARS-CoV and human CoV-OC43 (HCoV-OC43) was investigated by peptide spot assay. RESULTS: Ninety-five of 142 residents (67%) and 53 of 160 staff members (33%) experienced symptoms of respiratory infection. Symptomatic residents experienced cough (66%), fever (21%) and pneumonia (12%). Eight residents died, six with pneumonia. No staff members developed pneumonia. Findings on reverse transcriptase-polymerase chain reaction assays for SARS-CoV at a national reference laboratory were suspected to represent false positives, but this was confounded by concurrent identification of antibody to N protein on serology. Subsequent testing by reverse transcriptase-polymerase chain reaction confirmed HCoV-OC43 infection. Convalescent serology ruled out SARS. Notably, sera demonstrated cross-reactivity against nucleocapsid peptide sequences common to HCoV-OC43 and SARS-CoV. CONCLUSIONS: These findings underscore the virulence of human CoV-OC43 in elderly populations and confirm that cross-reactivity to antibody against nucleocapsid proteins from these viruses must be considered when interpreting serological tests for SARS-CoV.