Hsa_circ_0065217 promotes growth and metastasis of renal cancer through regulating the miR-214-3p-ALPK2 axis. Issue 23 (2nd December 2021)

• Record Type: Journal Article
• Authors: Yan, Jia-sheng; Chen, Qi; Li, Ya-Lin; Gao, Yun-qiu
• Is Part Of: Cell cycle. Volume 20:Issue 23(2021)
• Page Start: 2519
• Page End: 2530
• Publication Date: 2021-12-02
• Subjects: Renal cell carcinoma -- hsa_circ_0065217 -- miR-214-3p -- ALPK2; Cell cycle -- Periodicals; 571.84377
• DOI: 10.1080/15384101.2021.1991123
• Languages: English
• ISSNs: 1538-4101

Abstract

ABSTRACT: Circular RNA (circRNA) deregulation impacts on normal cell physiology leading to malignant phenotypic changes. Here, we determined the function of the circRNA, hsa_circ_0065217 in malignant renal cell carcinoma (RCC). Hsa_circ_0065217 was abundantly expressed in RCC tissue and cell lines, and its expression linked to advanced TNM stages, large tumor sizes, and lymph-node metastasis. Hsa_circ_0065217 silencing reduced in vitro RCC cell-line growth and aggressiveness. Mechanistically, hsa_circ_0065217 promoted alpha protein kinase 2 (ALPK2) expression via its competing endogenous RNA (ceRNA) activity toward miR-214-3p. Moreover, ALPK2 overexpression reversed hsa_circ_0065217 knockdown effects on RCC cell-line malignancy. Thus, hsa_circ_0065217/miR-214-3p/ALPK2 signaling putatively promotes RCC tumorigenesis and is a putative RCC treatment target.