Heterogeneity of immune cells in human atherosclerosis revealed by scRNA-Seq. Issue 13 (3rd August 2021)

• Record Type: Journal Article
• Authors: Vallejo, Jenifer; Cochain, Clément; Zernecke, Alma; Ley, Klaus
• Is Part Of: Cardiovascular research. Volume 117:Issue 13(2021)
• Page Start: 2537
• Page End: 2543
• Publication Date: 2021-08-03
• Subjects: Atherosclerosis -- scRNA-Seq -- Transcriptomes -- Antibodies -- Human; Cardiovascular system -- Diseases -- Periodicals; Cardiovascular system -- Periodicals; 616.1
• DOI: 10.1093/cvr/cvab260
• Languages: English
• ISSNs: 0008-6363

Abstract

Abstract: Immune cells in atherosclerosis include T, B, natural killer (NK) and NKT cells, macrophages, monocytes, dendritic cells (DCs), neutrophils, and mast cells. Advances in single-cell RNA sequencing (sRNA-Seq) have refined our understanding of immune cell subsets. Four recent studies have used scRNA-Seq of immune cells in human atherosclerotic lesions and peripheral blood mononuclear cells (PBMCs), some including cell surface phenotypes revealed by oligonucleotide-tagged antibodies, which confirmed known and identified new immune cell subsets and identified genes significantly up-regulated in PBMCs from HIV + subjects with atherosclerosis compared to PBMCs from matched HIV + subjects without atherosclerosis. The ability of scRNA-Seq to identify cell types is greatly augmented by adding cell surface phenotype using antibody sequencing. In this review, we summarize the latest data obtained by scRNA-Seq on plaques and human PBMCs in human subjects with atherosclerosis.