Computer-aided design of magnetic molecularly imprinted polymer nanoparticles for solid-phase extraction and determination of levetiracetam in human plasma. Issue 26 (17th April 2018)

• Record Type: Journal Article
• Authors: Attallah, Olivia A.; Al-Ghobashy, Medhat A.; Ayoub, Ahmed Taha; Tuszynski, Jack Adam; Nebsen, Marianne
• Is Part Of: RSC advances. Volume 8:Issue 26(2018)
• Page Start: 14280
• Page End: 14292
• Publication Date: 2018-04-17
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c8ra02379d
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : A novel molecularly imprinted polymer on Fe3 O4 nanoparticles was applied to extract antiepileptic drug; levetiracetam from plasma for TDM purposes. Abstract : Analytical methods should be accurate and specific to measure plasma drug concentration. Nevertheless, current sample preparation techniques suffer from limitations, including matrix interference and intensive sample preparation. In this study, a novel technique was proposed for the synthesis of a molecularly imprinted polymer (MIP) on magnetic Fe3 O4 nanoparticles (NPs) with uniform core–shell structure. The Fe3 O4 @MIPs NPs were then applied to separate and enrich an antiepileptic drug, levetiracetam, from human plasma. A computational approach was developed to screen the functional monomers and polymerization solvents to provide a suitable design for the synthesized MIP. Different analysis techniques and re-binding experiments were performed to characterize the Fe3 O4 @MIP NPs, as well as to identify optimal conditions for the extraction process. Adsorption isotherms were best fitted to the Langmuir model and adsorption kinetics were modeled with pseudo-second-order kinetics. The Fe3 O4 @MIP NPs showed reasonable adsorption capacity and improved imprinting efficiency. A validated colorimetric assay was introduced as a comparable method to a validated HPLC assay for the quantitation of levetiracetam in plasma in the range of 10–80 μg mL −1 after extraction. The results from the HPLC and colorimetric assays showed good precision (between 1.08% and 9.87%) and recoveries (between 94% and 106%) using the Fe3 O4 @MIP NPs. The limit of detection and limit of quantification were estimated to be 2.58 μg mL −1 and 7.81 μg mL −1, respectively for HPLC assay and 2.32 μg mL −1 and 7.02 μg mL −1, respectively for colorimetric assay. It is believed that synthesized Fe3 O4 @MIP NPs as a sample clean-up technique combined with the proposed assays can be used for determination of levetiracetam in plasma.