Effects of intracerebroventricular administration of irisin on the hypothalamus–pituitary–gonadal axis in male rats. Issue 6 (14th October 2018)

• Record Type: Journal Article
• Authors: Tekin, Suat; Beytur, Ali; Erden, Yavuz; Beytur, Asiye; Cigremis, Yilmaz; Vardi, Nigar; Turkoz, Yusuf; Tekedereli, Ibrahim; Sandal, Suleyman
• Is Part Of: Journal of cellular physiology. Volume 234:Issue 6(2019:Jun.)
• Page Start: 8815
• Page End: 8824
• Publication Date: 2018-10-14
• Subjects: FSH -- GnRH -- irisin -- LH -- testosterone; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.27541
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract: Irisin is a product of fibronectin type III domain‐containing protein 5 (FNDC5) and plays an important role in energy homeostasis. In this study, we aimed to determine effects of intracerebroventricular administration of irisin on the hypothalamus–pituitary–gonadal axis by molecular, biochemical, and morphological findings. Fourty male Wistar‐Albino rats were used and divided into four groups including control, sham (vehicle), 10, and 100 nM irisin infused groups ( n = 10). Hypothalamic gonadotropin releasing hormone (GnRH) level and serum luteinizing hormone (LH), follicle‐stimulating hormone (FSH), and testosterone levels were determined. Testicular tissue histology and spermiogram analysis were also performed. Both irisin concentrations significantly reduced hypothalamic GnRH messenger RNA (mRNA) and protein levels ( p < 0.05). It was found that serum LH, FSH, and testosterone levels and Sertoli and Leydig cell numbers were decreased by irisin administration ( p < 0.05). In addition, irisin administration reduced sperm density and mobility ( p < 0.05). However, it did not cause any change in testicular and epididymis weights and tubular diameter. Our results reveal that irisin can play a role in the central regulation of reproductive behavior and also reduces testosterone levels by suppressing LH and FSH secretion. These results suggest that the discovery of irisin receptor antagonists may be beneficial in the treatment of infertility. Abstract : Irisin infusion reduced hypothalamic gonadotropin releasing hormone (GnRH) messenger RNA (mRNA) and protein levels. Irisin infusion reduced serum luteinizing hormone (LH), follicle‐stimulating hormone (FSH), and testosterone levels. Irisin infusion reduced Sertoli and Leydig cell numbers. Irisin infusion reduced sperm density and mobility.