Caffeine versus antioxidant combination (Antox) and their role in modifying cadmium‐induced testicular injury in adult male albino rats. (28th December 2020)

• Record Type: Journal Article
• Authors: Labib, Heba; Galal, Ahmed
• Other Names: Agarwal Ashok guestEditor.; Baskaran Saradha guestEditor.; Durairajanayagam Damayanthi guestEditor.; Vij Sarah guestEditor.
• Is Part Of: Andrologia. Volume 53:Number 2(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-12-28
• Subjects: antox -- cadmium -- caffeine -- caspase‐9 -- StAR; Andrology -- Periodicals; Infertility, Male -- Periodicals; 616.69
• DOI: 10.1111/and.13948
• Languages: English
• ISSNs: 0303-4569

Abstract

Abstract: The aim of the current work was to compare the roles of caffeine and antioxidants in prevention of cadmium‐induced testicular damage when given, in addition to cadmium, in adult male albino rats. Histopathological and ultra‐structural examination as well as biochemical and molecular assessments were done. Cadmium chloride (4 mg/kg body weight) was administered via oral gavage from day 21 to 28 of the experiment. Caffeine (25 mg/kg) via intra‐peritoneal injection and antioxidant preparation (Antox) 10 mg/kg via oral gavage were given as a pre‐treatment for 21 days and concomitantly with Cd from day 21 to 28. Real‐time PCR was done for determination of 3, 17 β hydroxy steroid dehydrogenase steroidogenic acute regulatory protein, caspase‐9 and mitofusin 1, 2 gene expression. Testosterone level, glutathione S‐transferase enzyme activity, reactive oxygen species, malondialdehyde and superoxide dismutase were measured spectrophotometrically by ELISA. Histological and ultra‐structural evaluation revealed disturbance of normal architecture, vacuolisation and necrosis. Vascular dilatation and congestion and collagen fibre deposition were present. A statistically significant difference was seen in all parameters when caffeine and antioxidants were given against cadmium‐induced testicular injury. Overall, we conclude that both caffeine and antioxidants have the ability to reverse cadmium‐induced testicular injury when given as pre‐treatment prior to cadmium exposure.