Application of an immunoglobulin Y-alkaline phosphatase bioconjugate as a diagnostic tool for influenza A virus. Issue 1 (1st January 2019)

• Record Type: Journal Article
• Authors: Ozkan, Busra; Budama-Kilinc, Yasemin; Cakir-Koc, Rabia; Mese, Sevim; Badur, Selim
• Is Part Of: Bioengineered. Volume 10:Issue 1(2019)
• Page Start: 33
• Page End: 42
• Publication Date: 2019-01-01
• Subjects: Immunoglobulin Y -- influenza A -- alkaline phosphatase -- ELISA methods -- diagnosis; Biomedical engineering -- Periodicals; Biotechnology -- Periodicals; Microbiology -- Periodicals; 660.6
• DOI: 10.1080/21655979.2019.1586054
• Languages: English
• ISSNs: 2165-5987

Abstract

ABSTRACT: The diagnosis of influenza A virus is essential since it can be confused with influenza A like illness and lead to inaccurate drug prescription. In this study, the M2e peptide, a strategic antigen that is conserved in all virus subtypes, was used as a diagnostic marker of influenza A. For the first time, M2e-specific IgY antibody was covalently conjugated to alkaline phosphatase (ALP) enzyme in the presence of glutaraldehyde. The antibody-enzyme bioconjugate was characterized by fluorescence and Fourier-transform infrared spectroscopy. Subsequently, the diagnostic value of this bioconjugate was evaluated by direct sandwich ELISA using nasopharyngeal swab samples positive/negative for H1N1 and H3N2, which were previously analyzed by rRT-PCR for influenza. In conclusion, the M2e-specific IgY-ALP bioconjugate demonstrated positive results for Influenza A in samples that were diagnosed as Influenza A via the RT-PCR method.