Effect of Gpx3 gene silencing by siRNA on apoptosis and autophagy in chicken cardiomyocytes. Issue 6 (4th December 2018)

• Record Type: Journal Article
• Authors: Gong, Yafan; Yang, Jie; Cai, Jingzeng; Liu, Qi; Zhang, Jun min; Zhang, Ziwei
• Is Part Of: Journal of cellular physiology. Volume 234:Issue 6(2019:Jun.)
• Page Start: 7828
• Page End: 7838
• Publication Date: 2018-12-04
• Subjects: apoptosis -- autophagy -- cardiomyocytes -- chickens -- glutathione peroxidase 3; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.27842
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract: Glutathione peroxidase 3 (Gpx3), as an important selenoprotein, is the most crucial antioxidant defense in cardiomyocytes. However, the role of Gpx3 in Se‐deficient cardiomyocyte damage still less reported. Here, we developed Gpx3 silence cardiomyocytes culture model (small interfering RNA; siRNA) for research the crosstalk between autophagy and apoptosis. Quantitative real‐time PCR and western blot analysis are performed to detect the expression of apoptosis and autophagy‐related genes. MDC stain, flow cytometry, AO/EB stain, and electron microscope were performed to observe the changes of cell morphology. Our results reveal that Gpx3 suppression can significant increases in ROS ( p < 0.05) levels, which further induced apoptosis through upregulated the expression of Caspase‐3 in cardiomyocytes. Meanwhile, we also found that the whole process is accompanied by the occurrence of autophagy, which are promoted by inhibiting the mTOR, and increasing the expression of ATG‐7, ATG‐10, and ATG‐12. Altogether, we conclude that the apoptotic and autophagic response machineries share antagonistic function in Gpx3 knockdown cardiomyocytes. Abstract : 1. Developed glutathione peroxidase (Gpx3) silence cardiomyocytes model. 2. Gpx3 silence can induce autophagy and apoptosis in chicken cardiomyocytes. 3. Apoptotic and autophagic response machineries share antagonistic function in Gpx3 knockdown cardiomyocytes.