The response regulator ArcA enhances biofilm formation in the vpsT manner under the anaerobic condition in Vibrio cholerae. (July 2020)

• Record Type: Journal Article
• Authors: Xi, Daoyi; Yang, Shuang; Liu, Qian; Li, Yujia; Li, Yuehua; Yan, Junxiang; Wang, Xiaochen; Ning, Kexin; Cao, Boyang
• Is Part Of: Microbial pathogenesis. Volume 144(2020)
• Publication Date: 2020-07
• Subjects: Vibrio cholerae -- Low oxygen -- Two component system -- Biofilm; TCS two component system -- wt wild type strain -- LB Luria-Bertani -- qPCR quantitative real-time polymerase chain reaction -- CI competitive index -- CT cholera toxin -- TCP toxin co-regulated pilus; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2020.104197
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Vibrio cholerae, the agent of severe diarrheal disease cholera, is known to form biofilm to persist in the environmental and the host, s intestines. The bacteria execute a complex regulatory pathway producing virulence factors that allow colonization and cause disease in response to environmental signals in the intestine, including low oxygen-limited condition. VpsR and VpsT are primary regulators of the biofilm formation-regulatory network. In this study, we determined that anaerobic induction enhanced biofilm formation via the two component system, ArcB/A, which functions as a positive regulator of toxT expression. The biofilm formation has reduced approximately 2.4-fold in the Δ arcA mutant compared to the wild type in anaerobic condition. Chip-qPCR and EMSA assays confirmed that ArcA can bind directly to the vpsT promoter and then activates the expression of biofilm formation related genes, vpsA - K and vps L - Q . Meanwhile, the Δ arcA mutant decreased the ability of colonization in intestine with CI (competition index) of 0.27 compared to wild type strain. These results suggest that ArcA links the expression of virulence and biofilm synthesis genes during anaerobic condition, and contributes to understand the complex relationship between biofilm formation and the intestinal signals during infection. Highlights: Low oxygen can stimulate biofilm formation in V. cholerae E12382. The response regulator ArcA can enhance biofilm formation by binding directly to the vpsT promoter in V. cholerae E12382. Inactivation of arcA gene in V. cholerae E12382 decreased the ability of colonization in the intestine.