Addition of caffeine to equine thawed sperm increases motility and decreases nitrite concentration. (28th December 2020)
- Record Type:
- Journal Article
- Title:
- Addition of caffeine to equine thawed sperm increases motility and decreases nitrite concentration. (28th December 2020)
- Main Title:
- Addition of caffeine to equine thawed sperm increases motility and decreases nitrite concentration
- Authors:
- Alves, Natália de Castro
Diniz, Soraia de Araújo
Viegas, Rodrigo Novaes
Cortes, Steyner Franca
Costa, Eduardo Damasceno
Freitas, Marina Morra
Martins‐Filho, Olindo Assis
Araújo, Márcio Sobreira Silva
Lana, Ângela Maria Quintão
Wenceslau, Raphael Rocha
Lagares, Monique de Albuquerque - Other Names:
- Agarwal Ashok guestEditor.
Baskaran Saradha guestEditor.
Durairajanayagam Damayanthi guestEditor.
Vij Sarah guestEditor. - Abstract:
- Abstract: The aim of this study was to improve the quality of frozen‐thawed equine sperm by the addition of caffeine to it. Semen from nine stallions was frozen and different concentrations of caffeine (3, 5 and 7.5 mM) were added to frozen‐thawed semen. The sperm kinetic parameters, membrane functionality and integrity, and acrosome integrity and spontaneous acrosome reacted sperm were evaluated with a computer‐assisted sperm analysis, a hypoosmotic swelling test and epifluorescent microscopy, respectively. Nitrite and hydroperoxide concentrations of frozen‐thawed semen were measured using spectrophotometry. Sperm fertility was evaluated by artificial insemination (AI) of 16 mares with thawed ejaculates (control and 5 mM caffeine‐treated groups). Compared to that in the control, the addition of 5 mM caffeine induced an increase in sperm motility (38.9 ± 2.8 versus 32.6 ± 3.4%), and a decrease in nitrite concentration (11.4 ± 2.1 versus 12.8 ± 2.9 µM/µg protein, p < .05). Moreover, the pregnancy rate from AI in the caffeine group was significantly higher (62.5%) than that in the control group (12.5%). These data suggest that caffeine reduced the nitrite concentration and enhanced sperm motility in thawed equine sperm, thus increasing the fertility rate in mares inseminated with caffeine‐treated equine semen.
- Is Part Of:
- Andrologia. Volume 53:Number 2(2021)
- Journal:
- Andrologia
- Issue:
- Volume 53:Number 2(2021)
- Issue Display:
- Volume 53, Issue 2 (2021)
- Year:
- 2021
- Volume:
- 53
- Issue:
- 2
- Issue Sort Value:
- 2021-0053-0002-0000
- Page Start:
- n/a
- Page End:
- n/a
- Publication Date:
- 2020-12-28
- Subjects:
- cryopreservation -- semen -- sperm motility -- sperm nitrite -- stallion
Andrology -- Periodicals
Infertility, Male -- Periodicals
616.69 - Journal URLs:
- https://onlinelibrary.wiley.com/journal/14390272 ↗
https://www.hindawi.com/journals/and/ ↗
http://onlinelibrary.wiley.com/ ↗ - DOI:
- 10.1111/and.13918 ↗
- Languages:
- English
- ISSNs:
- 0303-4569
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 0900.443000
British Library DSC - BLDSS-3PM
British Library HMNTS - ELD Digital store - Ingest File:
- 25869.xml