MiR‐30e‐5p suppresses cell proliferation and migration in bladder cancer through regulating metadherin. Issue 9 (8th May 2019)

• Record Type: Journal Article
• Authors: Zhang, Zhenxing; Qin, Haixiang; Jiang, Bo; Chen, Wei; Cao, Wenmin; Zhao, Xiaozhi; Yuan, Hui; Qi, Wei; Zhuo, Dong; Guo, Hongqian
• Is Part Of: Journal of cellular biochemistry. Volume 120:Issue 9(2019)
• Page Start: 15924
• Page End: 15932
• Publication Date: 2019-05-08
• Subjects: bladder cancer -- migration -- miR‐30e‐5p -- metadherin -- proliferation; Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.28866
• Languages: English
• ISSNs: 0730-2312

Abstract

Abstract: Recent studies have suggested that miR‐30e‐5p is dysregulated in several human carcinomas; however, the mechanism of miR‐30e‐5p in bladder cancer (BCa) remains unknown. Here, we confirmed that the expression of miR‐30e‐5p was decreased in human BCa specimens and cell lines by quantitative reverse‐transcription polymerase chain reaction (qRT‐PCR). Upregulation of miR‐30e‐5p decreased the proliferation and migration in T24 and UM‐UC‐3 cells. Metadherin (MTDH) was a potential target for miR‐30e‐5p through bioinformatics analysis. Dual‐luciferase assays were conducted to validate the interaction between miR‐30e‐5p and MTDH, which demonstrates that the relative luciferase activity was significantly downregulated after transfected miR‐30e‐5p mimic compared with control mimic in 293T cells. We also detected that whether silencing of MTDH by using small interfering(si)‐MTDH matched effects caused by miR‐30e‐5p overexpression in BCa cells lines by Cell Counting Kit‐8 (CCK‐8), colony formation, and transwell assay, and we found the effects of silencing of MTDH same as miR‐30e‐5p overexpression. Furthermore, we verified that the restoration of MTDH in miR‐30e‐5p‐overexpressed BCa cells rescued the inhibitory effects of miR‐30e‐5p. In conclusion, these results demonstrated that miR‐30e‐5p may inhibit BCa cells growth and invasiveness by targeting MTDH and may be a promising therapeutic agent for treating clinical BCa patients. Abstract : It is the first time that miR‐30e was investigated in clinical bladder cancer (BCa) tissues and BCa cells. We detected the functions of miR‐30e and metadherin (MTDH) in BCa cells. Furthermore, we elucidated the relationship between them. miR‐30e‐5p and its downstream target MTDH could be considered as diagnosed biomarker and promising therapeutic target for BCa in the future time.