Isolation and polyphasic identification of Tateyamaria armeniaca sp. nov. Issue 15 (22nd August 2019)

• Record Type: Journal Article
• Authors: Yoon, Jaewoo
• Is Part Of: FEMS microbiology letters. Volume 366:Issue 15(2019)
• Publication Date: 2019-08-22
• Subjects: Alphaproteobacteria -- Tateyamaria armeniaca sp. nov -- seawater -- 16S rRNA gene -- polyphasic taxonomy; Microbiology -- Periodicals; 579
• DOI: 10.1093/femsle/fnz182
• Languages: English
• ISSNs: 0378-1097

Abstract

ABSTRACT: A novel alphaproteobacterium, designated KMU-156 T, was isolated from seawater collected on the coast of Jeju Island, Republic of Korea, and its phylogenetic position was determined using a polyphasic taxonomic approach. Strain KMU-156 T was Gram-stain-negative, strictly aerobic, apricot-colored, rod-shaped, non-motile and chemoorganoheterotrophic. Phylogenetic study based on the 16S rRNA gene sequence revealed that the novel bacterium belongs to the family ' Rhodobacteraceae ', of the class Alphaproteobacteria, and that it possessed the greatest sequence similarity (98.2%) with Tateyamaria omphalii MKT107 T . DNA–DNA hybridization values between strains KMU-156 T, T. omphalii KCTC 12333 T and Tateyamaria pelophila DSM 17270 T were less than 70%. The major isoprenoid quinone of the novel isolate was ubiquinone-10 (Q-10) and the major (> 10%) cellular fatty acids were C16:0 and C18:1 ω 7 c . The genomic DNA G + C content of strain KMU-156 T was 59.3 mol%. The polar lipid profile of the strain KMU-156 T had phosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid, an unidentified phospholipid and two unidentified lipids. From the discriminative taxonomic features, the strain is considered to represent a novel species of the genus Tateyamaria for which the name Tateyamaria armeniaca sp. nov. is proposed. The type strain of T. armeniaca sp. nov. is KMU-156 T (= KCCM 90321 T = NBRC 113460 T ). Abstract : On the basis of differential phylogenetic and phenotypic evidence, the novel strain KMU-156 T represents a novel species of the genus Tateyamaria, T. armeniaca sp. nov.