Green and chemically synthesized zinc oxide nanoparticles: effects on in-vitro seedlings and callus cultures of Silybum marianum and evaluation of their antimicrobial and anticancer potential. Issue 1 (1st January 2021)

• Record Type: Journal Article
• Authors: Saeed, Faryal; Younas, Muhammad; Fazal, Hina; Mushtaq, Sadaf; Rahman, Faiz ur; Shah, Muzamil; Anjum, Sumaira; Ahmad, Nisar; Ali, Mohammad; Hano, Christophe; Abbasi, Bilal Haider
• Is Part Of: Artificial cells, nanomedicine, and biotechnology. Volume 49:Issue 1(2021)
• Page Start: 450
• Page End: 460
• Publication Date: 2021-01-01
• Subjects: Silybum marianum -- ZnO-NPs -- biochemical profile -- anti-cancer -- antimicrobial -- antioxidant; Artificial cells -- Periodicals; Nanotechnology -- Periodicals; Blood substitutes -- Periodicals; Tissue engineering -- Periodicals; Molecules -- Periodicals; Biotechnology -- Periodicals; 615.39
• DOI: 10.1080/21691401.2021.1926274
• Languages: English
• ISSNs: 2169-1401

Abstract

Abstract: Zinc oxide nanoparticles (ZnO-NPs) have been produced by physical and chemical methods. Here, the comparative evaluation of both chemically-synthesised ZnO-NPs (C-ZNPs) and in-vitro cultured S. marianum mediated green-synthesised ZnO-NPs (G-ZNPs) were investigated on seed germination frequency, root and shoot growth, callus induction and biochemical profile of medicinally important plant Silybum marianum . Of all the treatments, callus-mediated ZnO-NPs gave optimum results for seed germination (65%), plantlet's root length (4.3 cm), shoot length (5.3 cm) and fresh and dry weights (220.4 g L −1 and 21.23 g L −1, respectively). Similarly, the accumulation of phenolic (12.3 µg/mg DW) and flavonoid (2.8 µg/mg DW) contents were also enhanced in callus cultures treated with G-ZNPs. We also observed maximum antioxidant activity (99%) in callus cultures treated with G-ZNPs, however, in case of plantlets, these activities were found highest for in-vitro whole plant-mediated ZnO-NPs. Moreover, G-ZNPs also enhanced total protein content (265.32 BSAE/20g FW) in callus cultures. G-ZNPs were further assessed for their effects on several multidrug resistant bacterial strains and human liver carcinoma (HepG2) cells and our findings revealed that callus extracts treated with G-ZNPs show ameliorated antibacterial (highest zone of inhibition (19 mm) against Klebsiella pneumonia ) and anticancer (highest cytotoxicity of 64%) activities.